Volume 13, Issue 1 (10-2018)                   MGj 2018, 13(1): 79-89 | Back to browse issues page

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Etminan A, Mehrabi A, Shooshtari L, Moradkhani H. Applicability of CBDP markers to study of genetic diversity among some of the cultivated wheat accessions and their ancestral species. MGj. 2018; 13 (1) :79-89
URL: http://mg.genetics.ir/article-1-45-en.html
Abstract:   (802 Views)
The knowledge about genetic diversity in the wild relatives of wheat provides useful information for breeding programs and gene pool management. In the present study, the genetic diversity among some of the cultivated wheat accessions and wild relatives belonging to T. aestivum, T. durum, T. urartu, Ae. tauschii and Ae. speltoides species was evaluated using 15 CBDP primers. A total of 141 bands amplified, which all of them were polymorphic. The average of polymorphic information content (PIC = 0.48) and resolving power (Rp = 10.67) revealed high efficiency of these markers to further genetic diversity assay. Furthermore, the average percentage polymorphic loci (PPL), number of observed (Na) and effective (Ne) alleles, Shannon’s information index (I), and gene diversity (H) are 72.48%, 1.44, 1.64, 0.38 and 0.26, respectively. These were coincided in essence with the analysis of molecular variance (AMOVA) results, indicating that 63 and 37% of genetic variation were found within different species, respectively. Genetic relationships inferred from a nighbour-joining dendrogram clustered accessions into main three groups. This grouping pattern was matched with the groups obtained by principal coordinate analysis (PCoA), revealing all accessions grouped based on their genomic constitution. Taken together, our results suggest CBDP markers will be useful for genetic diversity and phylogenetic studies in the domesticated and wild relatives of wheat. Hence, the use of this technique in other breeding programs such as, QTL mapping and construction of linkage maps are recommended.
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Type of Study: Applicable | Subject: Subject 01
Received: 2019/09/30 | Accepted: 2019/09/30 | Published: 2019/09/30

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