Jahangiri M, Saffar B, Kharazian N, Latifi navid S, Zahri S. The study of chloroplast genes matK, rbcL and trnL-F in Salvia nemorosa L. and Thymus syriacus Boiss. MGj 2019; 13 (4) :537-542
URL:
http://mg.genetics.ir/article-1-88-en.html
Abstract: (1721 Views)
The plant family Lamiaceae contains several species with potential therapeutic activity due to their essential oils. In this study, the chloroplast genes of two species of the Lamiaceae family, Thymus syriacus and Salvia nemorosa, were sequenced to make the identification of these two species quickly and accurately. The genes matK and rbcL were used for sequencing Thymus syriacus and matK, trnL-F for Salvia nemorosa. At first, genomic DNA was extracted from the leaves of these two species, then the sequences of the desired chloroplast genes were amplified and eventually sequenced. The sequence of the obtained chloroplast genes can be used in other phylogenetic and evolutionary studies to compare the species and could therefore provide more accurate results within a shorter time especially in large-scale studies. The results shows that chloroplast regions as matK, rbcL and trnL-F can be used as DNA barcodes for identification of Salvia nemorosa and Thymus syriacus species.
Type of Study:
Research |
Subject:
Subject 01 Received: 2016/08/12 | Accepted: 2019/03/6 | Published: 2019/10/2