Showing 3 results for Farhadi
S Farhadi, Ms Sabet, A Moieni, Ma Malbobi,
Volume 15, Issue 1 (7-2020)
Abstract
The availability of nutrients such as phosphorus in plant, in addition to plant yield increment, could increase growth rate and thus reduce plant growth period. Therefore, identification and overexpression of genes involved in nutrient uptake are the important steps to increase of yield and tolerance to environmental stresses particularly the end seasons stresses. Purple acid phosphatases, one of the most important phosphatase family, play a key role in increasing phosphorus availability in plants. In this study, the effect of knock-out and overexpression of AtPAP17 and AtPAP26 genes (two important members of Arabidopsis thaliana purple acid phosphatase family) on some physiological and phonological traits of Arabidopsis thaliana was investigated under Pi-sufficient (1.25 mM KH2PO4) and Pi-deficient (0 mM KH2PO4) conditions. For this purpose, single and double mutant plants of AtPAP17 and AtPAP26 genes were studied in this research. To confirm the function of mentioned genes in mutant plants, transformed Arabidopsis plants containing the CaMV-35S:AtPAP17 and CaMV-35S:AtPAP26 constructs were studied as overexpressed plants of AtPAP17 and AtPAP26 genes, respectively. The result showed that biomass and phosphorus content of plants increased in single mutant plants as compared to double mutant plants under Pi-sufficient condition. Superiority of single mutant plants as compared to double mutant plants indicated a remarkable contribution of AtPAP17 and AtPAP26 genes in phosphatase compensation network. Our results showed at least one of these genes (AtPAP17 and AtPAP26) activity is essential for increasing phosphorus availability and fast growth in Arabidopsis thaliana. Also, overexpressed plants displayed a significant increase in phosphorus content, flowering percentage, and biomass as compared to WT plants (Col-0). The results of this study showed that AtPAP17 and AtPAP26 genes could be important candidates for developing the plants with high ability of nutrient uptake, especially phosphorus, thereby reducing plant growth period.
Mohadeseh Ebrahimi, Mahyar Grami, Elham Younesi Melerdi, Ayoub Farhadi,
Volume 17, Issue 2 (7-2022)
Abstract
The objective of the present study is to investigate the response of halophyte Aeluropus littoralis to salinity stress. This experiment was conducted in three levels of treatments containing control (without salt), 200 and 400 mM NaCl with three replications for each. The changes were measured at 6 and 24 h after applying salt treatment. The results showed that the soluble sugar content increased with increasing time and NaCl concentration. Also, The proline content also increased significantly in the both concentrations of NaCl 24 h compared to the 6 h after salt stress. On the other hand, protein content at 6 h and 200 mM concentration was no significantly different, but there was a significant decrease in protein content at 400 Mm treatment at the same time. In contrast, the protein content was significantly decreased in both concentrations of NaCl 24 h after treatment. The results of relative gene expression analysis showed that PYL gene expression was strongly affected by salinity stress and showed a significant decreasing trend (p <0.05). So that, the rate of this reduction at 6 hours is much higher than at 24 hours after salt treatment. ABF gene expression generally showed an increasing trend, which increased significantly in both treatments after 24 h. Therefore, it can be stated that soluble sugars and proline as adaptive compounds in response to salinity stress in Aeluropus littoralis plant and abscisic acid signaling pathway are strongly affected by salinity stress.
Mrs Narges Ghasemi Jouybari, Alamara Gholami, Ayoub Farhadi, Elham Yunesi-Melerdi,
Volume 19, Issue 1 (5-2024)
Abstract
The objective of this investigation was to compare the relative expression level of the IRAK1 and TRAF6 genes in the mammary tissue of cows afflicted with clinical mastitis and unaffected cows utilizing the Real-time PCR technique. For this particular purpose, a total of 15 mammary tissue samples, consisting of 5 healthy specimens and 10 samples with clinical mastitis. An RNA extraction kit was employed to extract RNA from the designated samples. Following the assessment of both the quality and quantity of the extracted RNA, it was utilized for cDNA synthesis. Subsequently, a pair of specific primers were employed twice to ascertain the relative expression level of the desired genes in relation to the I8SrRNA gene serving as an internal control through the qPCR technique. The relative expression level of the genes was determined using the 2-ΔΔct method, while the statistical discrepancy between the diseased and healthy groups was analyzed utilizing the t-student test in SAS statistical software version 9/1. The acquired outcomes indicated that the expression levels of the TRAF6 and IRAK1 genes in the diseased samples were 2.53 (P<0.024) and 3.04 (P<0.0012) times higher, respectively. The identification of solutions to develop strategies for the control of mastitis disease is of considerable significance, with the treatment thereof possessing substantial economic rationale for the global dairy cattle industry. The results obtained from this investigation may be employed to enhance comprehension pertaining to the role of TRAF6 and IRAK1 genes within the defense system of mammary glands, as well as to devise novel examinations geared towards the identification of polymorphisms within these genes for application in breeding programs aimed at identifying mastitis-resistant animals.
