%0 Journal Article %A مهدی رضایی, %A محمدرضا نقوی, %A عبدالهادی حسین زاده, %A علیرضا عباسی, %T Study the expression of genes involved in morphinan alkaloids biosynthesis and corresponding metabolites in Papaver somniferum %J Modern Genetics Journal %V 11 %N 2 %U http://mg.genetics.ir/article-1-1421-en.html %R %D 2016 %K Benzylisoquinoline Alkaloids, Codeine, Gene Expression, Morphine, Thebaine, %X Combined analysis of transcript and metabolite profiling data presents a new and meaningful approach in the identification of candidate genes for changing the metabolic composition of a biological system. Holistic understanding of the biological behavior of a complex system enables the careful tracking of the response of an organism to conditional perturbations at different molecular and genetic levels. The availability of species with specific genomic and metabolomics resources creates new opportunities to investigate the biosynthesis and regulation of alkaloid metabolism in opium poppy. The relationship between expression of genes which encode Thebaine-6-O-demethylase, Codeine-O-demethylase and Codeine Reductase enzymes and the amount of codeine and morphine as corresponding products, and thebaine as precursor of the pathway in different parts of adult plants were studied. The results for T6ODM showed the lowest expression in roots and the highest in the upper part of the stems. The lowest and highest levels of transcripts for CODM were observed in roots and leaves, respectively. Leaves and upper part of stem had the lowest and highest level of COR transcripts. The predominant alkaloid of roots was thebaine and leaves had the lowest amount of thebaine. Surprisingly, although morphine and codeine were not detected in the roots, while, capsules had the highest amount of the metabolites. Despite of coordination between alkaloids rates in different parts of plants, coordinate regulation among transcript levels and morphinan alkaloids rates were not observed. Although, capsules had the maximum levels of codeine and morphine, the levels of transcripts encoded biosynthetic enzymes which convert thebaine into codeine and morphine were not at maximum levels. Therefore presumably molecular, biochemical and cellular factors other than the transcript level affect the accumulation of metabolites in specific tissues. %> http://mg.genetics.ir/article-1-1421-en.pdf %P 229-236 %& 229 %! %9 %L A-10-109-421 %+ %G eng %@ 2008-4439 %[ 2016