Modern Genetics Journal
فصلنامه علمی ژنتیک نوین
MGj
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2008-4439
2008-4439
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2024
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بررسی بیان ژنهای IRAK1 و TRAF6 در بافت پستان گاوهای هلشتاین مبتلا به ورم پستان بالینی
Investigating the expression of IRAK1 and TRAF6 genes in the mammary tissue of Holstein cows with clinical mastitis
ژنتیک جانوری
Subject 02
پژوهشی کامل
Applicable
<span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">هدف از پژوهش حاضر بررسی مقایسه­ای میزان بیان نسبی ژن­های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">IRAK1</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">TRAF6</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> در بافت پستان گاوهای مبتلا به ورم پستان بالینی و گاوهای سالم با استفاده از تکنیک </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Real-time PCR</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> بوده است. نمونه­های مورد مطالعه 15 نمونه بافت پستان که شامل 5 نمونه سالم و 10 نمونه مبتلا به ورم پستان بالینی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">بود</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">. پس از بررسی کیفیت و کمیت </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">RNA</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> استخراج شده، از آن برای سنتز </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">cDNA</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> استفاده شد. سپس از دو جفت آغازگر اختصاصی برای اندازه­گیری میزان بیان نسبی ژن­های مورد نظر نسبت به ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">I8SrRNA</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> به عنوان ژن کنترل داخلی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">با تکنیک </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">qPCR</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> استفاده شد. میزان بیان نسبی ژن­ها با روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">2<sup>-ΔΔct</sup></span></span></span><sup> </sup><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">تعیین و تفاوت آماری بین گروه بیمار و سالم با آزمون </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">t-student</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> و در نرم­افزار آماری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">SAS</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> ویرایش 1/9</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> بررسی شد. نتایج به دست آمده نشان داد که </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">میزان بیان ژن­های </span></span></span><a name="_Hlk111153052"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">TRAF6</span></span></span></a><span style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">IRAK1</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:"> در نمونه­های بیمار نسبت به نمونه­های سالم به ترتیب 53/2 (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">P<024</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">) و 04/3 (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">P<0.0012</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">) برابر بیشتر بوده است. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">شناسایی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">راه</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">حل­هایی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">برای</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">توسعه</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">راهبردهای</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">کنترلی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">بیماری</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">ورم پستان،</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">از</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">اهمیت</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">زیادی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">برخوردار</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">بوده</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">و</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">درمان</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">آن</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">توجیه</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">اقتصادی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">قابل</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">توجهی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">برای</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">صنعت</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">پرورش</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">گاو</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">شیری</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">جهان</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">دارد. از نتایج این پژوهش</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">می­توان</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">برای</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">فهم</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">بهتر</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">نقش</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">ژنهای</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> TRAF6 </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">و</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> IRAK1</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">در</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">سیستم</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">دفاعی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">غدد</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">پستان و طراحی آزمایش­های جدید برای شناسایی چندشکلی­های موجود در این ژن­ها جهت استفاده از آن ها در برنامه­های اصلاح نژادی برای شناسایی دام­های مقاوم در برابر ورم پستان</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">استفاده</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">نمود</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The objective of this investigation was to compare the relative expression level of the IRAK1 and TRAF6 genes in the mammary tissue of cows afflicted with clinical mastitis and unaffected cows utilizing the Real-time PCR technique. For this particular purpose, a total of 15 mammary tissue samples, consisting of 5 healthy specimens and 10 samples with clinical mastitis. An RNA extraction kit was employed to extract RNA from the designated samples. Following the assessment of both the quality and quantity of the extracted RNA, it was utilized for cDNA synthesis. Subsequently, a pair of specific primers were employed twice to ascertain the relative expression level of the desired genes in relation to the I8SrRNA gene serving as an internal control through the qPCR technique. The relative expression level of the genes was determined using the 2<sup>-ΔΔct</sup> method, while the statistical discrepancy between the diseased and healthy groups was analyzed utilizing the t-student test in SAS statistical software version 9/1. The acquired outcomes indicated that the expression levels of the TRAF6 and IRAK1 genes in the diseased samples were 2.53 (P<0.024) and 3.04 (P<0.0012) times higher, respectively. The identification of solutions to develop strategies for the control of mastitis disease is of considerable significance, with the treatment thereof possessing substantial economic rationale for the global dairy cattle industry. The results obtained from this investigation may be employed to enhance comprehension pertaining to the role of TRAF6 and IRAK1 genes within the defense system of mammary glands, as well as to devise novel examinations geared towards the identification of polymorphisms within these genes for application in breeding programs aimed at identifying mastitis-resistant animals.</span></span></span></span></span><br>
ورم پستان بالینی, بیان ژن, گاو هلشتاین, qPCR
Clinical mastitis, Gene expression, Holstein cow, qPCR.
25
33
http://mg.genetics.ir/browse.php?a_code=A-10-440-1&slc_lang=fa&sid=1
Narges
Ghasemi Jouybari
نرگس
قاسمی جویباری
ghaseminarges433@gmail.com
10031947532846008588
10031947532846008588
No
Islamic Azad University, Sari Branch, Sari, Iran
دانشگاه آزاد اسلامی واحد ساری، ساری، ایران
Alamara
Gholami
عالم آرا
غلامی
alamaragholami.uni@gmail.com
10031947532846008589
10031947532846008589
No
Islamic Azad University, Sari Branch, Sari, Iran
دانشگاه آزاد اسلامی واحد ساری، ساری، ایران
Ayoub
Farhadi
ایوب
فرهادی
ayoubfarhadi60@gmail.com
10031947532846008590
10031947532846008590
Yes
Faculty of Animal Sciences and Fisheries, Sari Agricultural Scienculty oes and Ntural Resources University, Sari, Iran
دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
Elham
Yunesi-Melerdi
الهام
یونسی ملردی
eyonesi@gmail.com
10031947532846008591
10031947532846008591
No
Sari Agricultural Sciences and Ntural Resources University, Sari, Iran
دانشگاه علوم کشاورزی و منابع طبیعی ساری