Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Aggravation of cell division in Aloe vera calli treated by low level laser and its relation with secondary metabolites production
155
164
FA
منصور امیدی
مهسا توکلی
سیدعلی پیغمبری
هدیه پازکیان
شهریار عبدالحسینی
Life is basically subordinated to mitotic division and its regulations. The cell cycle is regulated by several mechanisms. Check points of the cell cycle are one of the most important regulatory mechanisms which make the cell to cease or proceed division. Phosphorylation and dephosphorilation cascade is another mechanism which triggers biochemical reactions. Therefore, MPF cytoplasmic factor, cyclin and cyclin dependent kinase play an essential role in signal transduction. In this study, the Aloe vera fresh calli were treated by He-Ne low level laser in 632.8 nm wavelength and 1-2mw power for 1, 3, 5 and 10 minutes. The calli area measured for 2 months after treatment. The results showed, low level laser affects cytochrome oxidase in the mitochondria electron transport chain and leads to energy production required for gene expression and synthesis of the necessary protein for cell division. Also using low level laser treatment, required phosphate for phosphorylation processes might be produced which by preventing cell death and accelerating cell divisions could increase callus area. The results of High Performance Liquid Chromatography showed a negative correlation between total amount of three secondary metabolites (Aloenin, Barbaloin and Iso-Barbaloin) and callus area. In recent study, the relation between cell division and accumulation of secondary metabolites was evaluated, in order to utilize low level laser as a low cost elicitor in the future researches.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Study of Ehd1 gene role in controlling flowering time in an advanced backcross population of rice at two climatic conditions
165
172
FA
اسماعیل شریف زاده
اسدالله احمدی خواه
بهرام ملکی زنجانی
Heading date is a key trait for geographical and seasonal adaptation of plants. It is a complex trait which is controlled by multiple genetic factors. In this research, the relationship of Ehd1 gene with heading date was assessed at two climatic conditions (Gorgan and Zanjan) in an advanced backcross population of rice derived from crossing two Iranian cultivars Neda and Sadri. For this purpose, 97 BC1F5 lines along with their parents were evaluated phenotypically at both locations and were genotyped using genetic primers specifically designed for the functional SNP of Ehd1. Phenotypic evaluations showed that in Zanjan with lower temperatures comparing to Gorgan, heading time delayed considerably. Molecular analysis of variance showed significant effect of functional SNP only in Gorgan. Association analysis showed that Ehd1 gene is related to 10.7% and 3.05% of phenotypic variations of heading date at two locations, respectively. Regarding to the decreasing effect of Ehd1I allele on rice heading date (-2.2 dayes), employing this allele in combination with the alleles of other genes with decreasing effect on heading date is applicable in marker-assisted selection (MAS) for improving early maturity in rice.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Software modeling of heat-shock protein 70 (HSP70) of poultry
173
183
FA
الهام رضوان نژاد
مجتبی مرتضوی
علی ریاحی مدواری
Heat shock proteins (HSPs) are the most of intracellular proteins. Their synthesis is rapidly up-regulated because of various ‘stressors’ including temperature, glucose deprivation, and infection. These proteins play an important role in survival cell and intracellular homeostasis under stress conditions. Since the crystallographic structure of the protein extensively damaged from heat stress it is not reported in poultry, modeling the structure of HSP70 of poultry can identify the mechanism of action of this protein against environmental stresses and help to reduce costs related to environmental stresses. In this study three-dimensional structure of protein HSP70 in poultry, species Gallus gallus was modeling to identify structural domains and specific position in this structure. The three-dimensional model was prepared by homology modeling using I-TASSER and Swiss Model with similar and suitable pattern from database PBD (Homo sapiens HSP70, Bovine HSC70). In order to evaluate and analysis of structure model SPDBV software was used. Quality evaluation of the model was done based on the structural parameters of C-score, TM-score and RMSD that Showed the quality of the proposed model is suitable and stable for HSP70 of poultry. Modeling HSP70 in poultry is the first stage in identifying of protein's structure of this chaperon. Due to the high similarity of the amino acid sequence of this Chaperon with determined structural Chaperons, this model can high reliability be used in later analysis and design.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
The effect of salinity in galactinol synthase (GAS) gene expression, antioxidant enzymes activity, carbohydrst and prolin in sistan melon landrace (Cucumis melo L.)
185
195
FA
فروزان حیدری
صالحه نادری
حمیده خواجه
عباسعلی بهاری
Salinity is a major problems in a wide area of Iran. Oligosaccharides of Raffinose family (RFOs) perform several physiological functions in plants such as accumulation of the suger in response to salt stress during seed forming. The synthesis of galactinol, mediated by galactinol synthase (GAS), is the first committed step in RFO formation. In order to resist to salinity, plants not only use osmotic regulation but also use a mechanism for increasing activity of antioxidant enzymes. In the present study, In order to determine GAS gene expression level, the role of catalase (CAT), ascorbate peroxidase (APX) and gayacol peroxidase (GPX), carbohydrat and proline levels in 3 selected Sistanian melon landraces (Cucumis melo L.) which are under salinity, a factorial arrangement in randomized complete block design with three replications experiment was done in Center of Agricultural Biotechnology of Zabol University. Four concentrations including 0, 50, 100 and 150 mM NaCl were used in this study. RNA was extracted from leaf samples and cDNA was designed using reverse transcription. The result showed that GAS gene expression increased by increasing salinity level from 0 to 150 mM. In comparison with control, in the highest level of salinity (150 Mm) GAS gene expression level increased 10.2-fold in Tashekandi landrace, 7.3-fold in Atashshirazi landrace and 19.79-fold in Sefidak landrace. The gene has the highest level of expression in Sefidak landrace comparing to other landraces. When salinity stress increased in three melon landraces of Tashekandi, Atashshirazi and Sefidak, antioxidant enzymes such as catalase (CAT) 85.9, 63.2 and 87.1 percent, ascorbate peroxidase (APX) 68.89, 36.48 and 92.81 percent, gayacol peroxidase (GPX) 89.25, 72.84 and 70.37 percent, carbohydrat 40.2, 84.4 and 92.8 percent and proline 86.6, 69.8 and 89.4 percent increased in comparison to control.It seems that expression of GAS gene synthesizes the sugars of raffinose family in three selected melon landraces in such condition which is effective for plant protection against the environmental stresses. This can help the plant for enduring under the salinity stress. It also provides the condition for plant endurance through the activity of two systems the enzyme activity increase and osmotic regulations.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Cytogenetic diversity in the populations of Coriandrum sativum L. of Iran
197
206
FA
نسترن اسماعیلی
حسین زینلی
آزاده صدرارحامی
منصوره صرامی
To study the cytogenetic diversity of 12 populations of Coriandrum sativum L. of Iran, squash technique and hematoxylin staining were used. In order to analyze the obtained Karyotype features, variance analysis in a completely randomized design with 3 replicates, the mean characteristics comparison using Duncan's test (at possibility level 1%), factor analysis with principal components analysis, cluster analysis in Ward method with standard Z-scores and analysis of variance comparing the characteristics mean between groups of cluster analysis, was performed. The results of Karyological study of tip root samples showed that basic chromosome number in all populations studied were x=11 and the number of chromosome were 2n = 2x = 22. In terms of Ploidy level, all populations were diploid. Among all total chromosome length (TL), long arm (LA), short arm (SA), difference between the relative length (DRL), relative length of the shortest chromosomes (S%) and the Asymmetry index between chromosomes (A2), there was a significant difference 1%. About percentage of the general shape of karyotype (TF%) and the relative length percentage of long arm (L% ) there was a significant difference in the level of 5%, which confirms the chromosomal diversity in studied populations. Cluster analysis of populations based on the characteristics of the karyotype were classified them into 3 groups. The greatest similarity was found between the two populations of Nishapur and Hamedan and the least similarity was found between the two populations of Ardebil, and Arak. The results of variance analysis of clusters showed that all traits except SA showed significant differences between the groups. Populations of Nishapur, Arak, Markazi, Mobarakeh, Najaf Abad and Hamedan in the first group in terms of CI, r-value, % TF and % S had the highest values that confirmed symmetrical karyotypes of this group. The populations Isfahan, Qazvin and Nahavand 2 in the second group in terms of characters were placed between the first and third levels. Populations of Malayer, Nahavand 1 and Ardebil in the third group in terms of the traits of chromosome length (TL, LA, SA, AR, DRL,% L) and indicators of asymmetry (A1, A2, AI) had the maximum values which shows the asymmetry karyotypes of this group.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Identifying Regulatory genes affected on Immune system by using Bos Taurus leukocyte RNA-Seq data
207
215
FA
الهام بهدانی
هدایت اله روشنفکر
مصطفی قادری زفره ای
جمال فیاضی
محمدرضا بختیاری زاده
Study of gene networks is one of the research fields that carried out on RNA-Seq data.The present study trying to identify effective genes on immune system and leukocytes activity by gene network of bovine leukocyte RNA-Seq data. In this research, gene network was studied by statistical GeneNet package related to R software, after identifying and measuring the expression level of genes and isoforms. Genes in this gene network that deal with them as regulator genes, often effect on some activities such as signaling pathways of leucocytes processes, cell destruction and infections, polarization of macrophages, migration and chemotaxis of leucocytes and neutrophils' phagocytosis. Considering to the key role of these genes in this gene networks, it suggests to searching about these genes' polymorphisms and evaluating relationship between different alleles and the immune responses. The best identified alleles could be used in gene assessed selection, microarray designing and genomic selection.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Effect of salt stress on expression pattern of miR160 and miR167 and their target genes in sunflower
217
227
FA
فهیمه خلجی
بهروز شیران
فریبا رفیعی
Salinity is a common environmental stress that seriously affects plant growth. The results of molecular researches in relation to environmental stresses showed that expression pattern of genes and their regulators were changed in response to stresses. miRNA's are an important class of gene expression regulators that diversely responds to environmental stresses and have a key role in post transcriptional regulation. In this study, the expression of miR160 and miR167 were studied by qRT-PCR at two salinity levels, (75 and 150 mM NaCl) and control condition in both root and leaf tissues. A factorial experiment based on completely randomized design(CRD) with three replication was used in this study. Plants were exposed to salinity stress in six-leaf stage and after 24 h sampling from leaf and root was performed then samples were freezed in liquid nitrogen. cDNA was synthesized with stem-loop RT-PCR procedure. Expression of miR160 and miR167 was measured using qRT-PCR technique. The results showed that the expression of miR160 was firstly increased and then decreased. While the values of fold change showed significant reduction of miR160 expression in 75mM salinity level, it didn’t show any significant differences comparing to control condition at 150 mM salinity. The expression of BU026935 gene in both salinity levels and tissues were increased in comparison to control. MiR167 was significantly down regulated at 75 mM salinity. The results of network analysis revealed that increasing miR167 expression under salinity condition is leading to auxin reduction. ARF6 expression level was decreased at 75mM salinity level in both root and leaf tissues. At 150 mM salinity level ARF6 gene showed down regulation only in root tissue.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Study the expression of genes involved in morphinan alkaloids biosynthesis and corresponding metabolites in Papaver somniferum
229
236
FA
مهدی رضایی
محمدرضا نقوی
عبدالهادی حسین زاده
علیرضا عباسی
Combined analysis of transcript and metabolite profiling data presents a new and meaningful approach in the identification of candidate genes for changing the metabolic composition of a biological system. Holistic understanding of the biological behavior of a complex system enables the careful tracking of the response of an organism to conditional perturbations at different molecular and genetic levels. The availability of species with specific genomic and metabolomics resources creates new opportunities to investigate the biosynthesis and regulation of alkaloid metabolism in opium poppy. The relationship between expression of genes which encode Thebaine-6-O-demethylase, Codeine-O-demethylase and Codeine Reductase enzymes and the amount of codeine and morphine as corresponding products, and thebaine as precursor of the pathway in different parts of adult plants were studied. The results for T6ODM showed the lowest expression in roots and the highest in the upper part of the stems. The lowest and highest levels of transcripts for CODM were observed in roots and leaves, respectively. Leaves and upper part of stem had the lowest and highest level of COR transcripts. The predominant alkaloid of roots was thebaine and leaves had the lowest amount of thebaine. Surprisingly, although morphine and codeine were not detected in the roots, while, capsules had the highest amount of the metabolites. Despite of coordination between alkaloids rates in different parts of plants, coordinate regulation among transcript levels and morphinan alkaloids rates were not observed. Although, capsules had the maximum levels of codeine and morphine, the levels of transcripts encoded biosynthetic enzymes which convert thebaine into codeine and morphine were not at maximum levels. Therefore presumably molecular, biochemical and cellular factors other than the transcript level affect the accumulation of metabolites in specific tissues.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Localization of QTLs conferring lodging resistance in barley recombinant inbred lines
237
244
FA
حسین احمدی اوچ تپه
حسن سلطانلو
سیده ساناز رمضان پور
محمدرضا نقوی
مهدی کلاته عربی
حمیدرضا نیک خواه
ساره یوسفی راد
Important agronomic traits have quantitative heritability and are controlled by many genes or QTLs. To locate QTLs controlling some important quantitative traits in barley, the present experiment was conducted with 162 recombinant inbred lines (RILs) derived from F8 generation plus the two parents (Arigashar and Igri) in an alpha-lattice design with three replications in Gorgan Agricultural Research Station in 2010. Quantitative traits inclusive plant height, peduncle length, spike length, and resistance to lodging were measured. Analysis of variance showed that all the traits of the lines were significantly different at one percent level. After extracting DNA from the young leaf sample, 21 SSR polymorphic primers and 13 AFLP polymorphic primers were evaluated on the individuals of the population. Linkage map with 542 centiMorgan (cM) including 105 SSR and AFLP markers were used for QTL analysis. QTL analysis using composite interval mapping (CIM) was performed. Totally, eight QTLs were detected including one for plant height, two for peduncle length, three for spike length and two for resistance to lodging, respectively. QLrgH2.1a at marker distance of 60.6 cM to Xbmag125 and QLrgH2.1b at marker distance of 72.6 cM to XE36-M49 on chromosome 2H (H2.1) are most likely QTLs for lodging resistance. The results of this experiment can be used in marker assisted selection (MAS) for production of lodging resistance varieties.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
A new gene involving in biofilm formation of Bacillus subtilis
245
259
FA
مریم خضری
مسعود احمد زاده
غلامرضا صالحی جوزانی
روح اله شریفی
R
esearcher has tented to focus on Bacillus subtilis due to having high potentials in biological control of plant diseases and also production of wide range of different metabolites. Biofilm production is known as one of the most important properties of this bacteria. In spite of huge studies performed on biofilm formation of this bacterium, mechanism of biofilm formation is not identified completely. So, the main objective of the present study was to explore new possible gene(s) involved in biofilm formation of B. subtilis. For this, mutation using mini-Tn10 was carried out in B. subtilis 168, and mutants without biofilm formation ability were selected. Comparing of mutated fragment sequence in mutant and wild type showed that the mautation has occurred in the ykuT gene. As the mutant could not form complete biofilm compared to the wild type strains, ykuT gene was introduced as one of the important genes in biofilm formation of B. subtilis for the first time in this study. The effect of different media on expression of ykuT gene was evaluated using FACs method. To do this, the promoter of the gene was inserted in the upstream region of the gfp gene in the pSG1151 and expression was studied. Our results indicated that Spizizen salt medium decreased the ykuT gene expression, whereas TYG, TYN and 2xSG media increased it. To evaluate the effects of ykuT gene on spoIIA (operon involved in sporulation) and hag (gene involved in motility) activities in B. subtilis, pSG1151 vectors containing spoIIA and hag promoters, were transferred to the wild type B. subtilis 168 and mutant strains (∆ykuT::spc). The results confirmed that the ykuT significantly affects the spoIIA and hag expression, as lack of ykuT expression caused silencing of the mentioned operon and gene. Comparison of sporulation and motility test between wild type and mutant, also photography using fluorescence microscopy confirmed the mentioned results.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Study of molecular dense markers associated with carcass and breast weight traits in Japanese quail
261
270
FA
حسین مهربان
نرگس مداحی
احمد احمدی
الهام رضوان نژاد
The objective of this study was to investigate the association of dense markers with breast and carcass weights in Japanese quail. Phenotypic data of breast (105 birds) and carcass weights (71 birds) that were selected for 4 week body weight were used as well as 12798 markers. Analyses of the data were done in three stages after editing the data. At first, the effect of markers was considered as fixed effects and removed non-significant markers. In second stage, the effect of significant markers were estimated in significant levels (1 to 5%) by using BayesCπ method, simultaneously. In third stage, the best significant levels were known, so bootstrap method was used to obtain markers with significant effect. Result showed that there are four markers affecting carcass weight significantly (P<0.05). Four significant markers for carcass and 10 significant markers for breast weight described 12.77% and 6.78% of phenotypic variance respectively. Only one of these significant markers was effective on both traits.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Evaluation of genetic diversity in salinity tolerance of wheat mutant lines using ISSR molecular marker
271
279
FA
آمنه قاسمی مصری
سعید نواب پور
احد یامچی
سعداله هوشمند
In order to evaluate effect of salt stress treatments on some biochemical traits in wheat genotypes (Trticum aestivum L.) an experiment was carried out under hydroponic condition. The experiment was performed as factorial in a completely randomized design with three replications. The treatments were the combination of two salinity levels (0 ds.m-1 (control) and 6 ds.m-1) and three wheat genotypes including, Tabasi cultivar and two mutant lines (T-67-60, T-65-7-1). Lipoxygenase enzyme assay (LOX), chlorophyll content and TBARM amount were measured. LOX, TBARM and chlorophyll levels showed significant differences among studied genotypes under the salinity conditions. Tabasi showed maximum level of LOX and TBARM content while minimum level belonged to T-67-60 line. Regarding to the fact that there is negative correlation between high level of TBARM and LOX amount with salt tolerance as well as positive correlation between high chlorophyll content with salinity tolerance, the most tolerance of T-67-60 mutant line is justified. Polymerase Chain Reaction (PCR) with 15 ISSR primers amplified about 182 bands. Among the 15 primers used in this study, 13 primers generated clear and reproducible banding patterns of which. Among them, six primers showed polymorphism. The number of fragments generated per primer varied from 13 (primer 17) to 22 (primer 15 and primer 22). The size of amplified fragments ranged from 100 to 3000 bp in all the primers. Fourteen polymorphic bands (7.6%) were observed among the amplified fragments, which were related to ISSR15, ISSR11, ISSR18, ISSR16, ISSR13 and ISSR27. ISSR18 showed the maximum (25%) of polymorphism. Genetic similarity between genotypes evaluated by Jaccard similarity coefficient ranged from 0.94 to 0.96. The low genetic distance between genotypes indicated a high degree of similarity between these genotypes. This results implicated that Tabasi is primarily the parent of the mutant lines. Diversity in the bands intensity and presence of some novel bands justified high rate of mutation in studied lines.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Locating genes associated with the roots of rice at seedling stage using recombinant lines under normal and drought conditions
281
292
FA
حسین صبوری
شریفه محمدآلق
In order to identify markers associated with genes controlling root traits related to drought tolerance under drought stress and normal conditions, 96 lines derived from crossing between Sepidroud and Anbarboo in hydroponic culture and Yoshida nutrient solution with -5 bar osmotic potential were evaluated. A Linkage map including 264 AFLP and 124 SSR markers was constructed that covered 1950.4 cM of rice genome. Average distance between markers was 5.20 cM. Length of shoot and root on 7th, 14th, 21th, 28th and 35th days after planting, shoot weight, root weight, thickness roots, biomass, leaf rolling and leaf frying code were recorded. qRT-2 (roots thickness on chromosome 2) under both condition stress and non-stress were identified. The detection of this QTL under the two different conditions, probably show that it is a stable QTL and environmental conditions did not have any influence on this QTL. Three major QTLs for 7th shoot length, root weight and root thickness between E120-M150-2 and E090-M160-3 markers on chromosome 3 were located under normal conditions. QTLs controlling root length at 7th (qRLD7-4b), qRM-2a and qRM-4 (root weight) under normal conditions, stem length on day 35 (qSLD35-9), leaf frying (qLF-6) and leaf rolling (qLR-6) were detected as major QTL under stress condition that these QTLs accounted for over 20 percent of the phenotypic variations. The markers showed tight linkage with the major QTLs in this research, could be used in marker-assisted selection breeding programs for selection of superior lines and incorporating of favorable alleles into commercial rice varieties.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Cloning and characterization of cyclotide-like genes in wheat
293
305
FA
سمیرا ترکمن
بهمن بهرام نژاد
جلال رستم زاده
نازنین کودری
C
yclotide-like genes have been previously reported from the data mining of the NCBI nucleotide database in the Poaceae family. In the current study, we have cloned new members of the cyclotide-like gene family from wheat cDNA using 3'RACE-PCR and specific primers based on conserved region of putative cyclotide-like sequences. PCR positive clones were randomly picked and sequenced. Eleven cyclotide-like sequences were identified. Phylogenetic analysis of nucleotide divided them in two classes and protein analysis showed that all sequences belong to two genes designated Tacycl1and Tacycl2. Length of these sequences was 402-479nt and showed high similarity with classical cyclotide-like genes in databases. Expressions of cyclotide-like gene Tacycl1 were studied in different organs, including leaves, flowers, stems and coleoptiles. Expression was significantly different in the organs; coleoptile had maximum relative expression and stems had minimum expression. In addition, expression of cyclotide-like gene Tacycl2 was studied following salicylic acid treatment. The expression of Tacycl1 increased 24h after salicylic acid treatment. This is the first report on the cloning of cyclotide-like genes in wheat.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Identification of polymorphism of G1 and G8 variants of GDF9 gene on multiple births Zel sheep
307
312
FA
لیلا شاه محمدی
مجتبی آهنی آذری
النا دهنوی
سعیده زره داران
فیروز صمدی
محمدرضا نصیری
رحمت سمیعی
علیرضا خان احمدی
سهیل یوسفی
The aim of this research was to investigate the polymorphism of prolificacy gene, GDF9 in Zel sheep breed using RFLP-PCR method and to study the association of genotypes with multiple birth records. Genomic DNA of 200 sheep was extracted using modified salting out extraction protocol to evaluate the mutations in G1 and G8 variants of GDF9 gene. Polymerase chain reaction was carried out to amplify a 462 and 139 bp fragments of GDF9 gene. The amplified fragments of G1 and G8 variants of GDF9 genes were digested with restriction enzymes Hin6I and DdeI, respectively. The G1 variant of GDF9 gene was polymorphic. Two mutant (B) and wild (A) type alleles with frequencies of 0.05 and 0.95 were detected in tested animals. Known's point mutation FecGH was monomorphic in tested animals. For G1 variant of GDF9 gene, a Chi-square test indicated that the population was in equilibrium. GLM procedure of SAS software was used to investigate the association of G1 variant of GDF9 gene with twining. Results indicated that there was no significant association (P>0.05) between polymorphism at this locus and twining.
Genetics Society
فصلنامه علمی ژنتیک نوین
2008-4439
11
2
2016
8
1
Genetic Diversity of Dendrostellera and Daphne genotypes Using ISSR Markers
313
318
FA
مصطفی علم هولو
سنبل ناظری
U
nderstanding of genetic diversity and classification of inheritance reserves (germplasm) are important and necessary activities in the management of preservation plant genetic reserves. In this study, the genetic diversity of twelve genotype of Daphne and Dendrostellera genera collected from 10 Iran Provinces, were analyzed using Inter Simple Sequence Repeat (ISSR) markers. 12 primers, which contained different simple sequence repeat (microsatellite), were used as single primer, and tested for PCR amplification. 88 polymorphic loci were scored. Cluster analysis method using the Jaccard coefficient with UPGMA method was assessed for genetic similarity among genotypes. The mean polymorphism information contents (PIC) and Marker Index (MI) were 0.86 and 6.45 respectively. P9 primer had the highest PIC (93%) and P6 primer had highest MI (11.04). The contribution of intragroups genetic diversity in total genetic diversity was estimated over than that of intergroups genetic diversity (0.85 versus 0.15). The results of this study revealed that ISSR markers could be efficiently used for genetic diversity of Daphne and Dendrostellera genotypes. Among used primers, the P9 primer with higher PIC is recommended for further analysis.