2024-03-29T16:29:31+03:30 http://mg.genetics.ir/browse.php?mag_id=142&slc_lang=fa&sid=1
142-1366 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Expression of gene encoding rice OsMTI-3a in E. coli ایمـان سلیمانی‌فـرد آذر شاه‌پیـری metallothioneins (MTs) are a group of low molecular weight and cystein-rich proteins that can bind metal ions through their thiol groups. In plant different MT isoforms are present. In this study, in order to study of the role of this protein, the coding sequence of gene encoding OsMTI-3a from rice (Oryza sativa), was cloned in pET41a and transferred into expression host, Escherichia coli strain Rosetta (DE3). After induction with IPTG, considerable amount of recombinant proteins was produced in the soluble fraction of the E.coli transformation. The tolerance of cells expressing recombinant proteins toward either Ni, Cu, Cd and Zn were compared to control by plotting their growth curve. In addition the amount of metal that was removed from medium or accumulated in the cells were determined using atomic adsorption spectroscopy (AAS). The results show that the isoform OsMTI-3a has ability to bind metals as follows: Cd>Ni>Zn>Cu. E. coli Heavy metal Expression Metallothionein Rice 2016 1 01 305 312 http://mg.genetics.ir/article-1-1366-en.pdf
142-1369 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Mapping genomic regions controlling some physiological traits associated with salt tolerance in recombinant inbred lines of wheat براتعلی فاخری سمیرا دباغ In order to determine the genomic regions of some physiological traits of "Seri M82×Babax" derived of 167 recombinent inbred line population of wheat under normal and salinity stress conditions, an experiment was conducted in two randomized alfa lattice design with two replications. Traits such as prolin content, water soluble carbohydrate, relative water content, chlorophyll a, chlorophyll b, chlorophyll c, Na+, K+ and Na+/K+ rario were measurerd. QTL analysis was carried out using genetic linkage map derived from 475 AFLP, SSR and DArT markers with total length of 1440.4 and the average distance of 3.03 cM and QTL cartographer 2.5 software with composite interval mapping method. Combined analysis of variance showed significant differences between lines for all studied traits. Maximum correlation was between chlorophyll b and chlorophyll c traits. In general, 23 QTLs were found for studied traits (10 and 13 QTLs for normal and stress conditions, respectively). Phenotypic variances that were explained by these QTLs changed from 6.80 to 15.65. The highest and lowest phenotypic variances were found for Na+/K+ ratio and chlorophyll c in the stress condition. LOD scores were ranged from 3.10 to 7.87. The highest and lowest LOD scores were attained for Na+/K+ ratio and water soluble carbohydrate in the stress condition. Q6ARWC of QTL controlling leaf relative water content, Q3APro controlling proline content, Q6AK controlling potassium concentration, Q4ANa/K controlling sodium to potassium ratio in different environments studied were present and had the necessary stability. So they might be used in marker assisted selection programs. Marker assisted selection for this trait may increase the resistance of plants to maintain growth in soils exposed to salt stress at critical stages of its growth. Physiological traits QTL Salinity stress Wheat 2016 1 01 313 328 http://mg.genetics.ir/article-1-1369-en.pdf
142-1367 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Aassociation of growth hormone promoter region with twining rate in Markhoz breed goats علیرضا عبدالمحمدی علی ویسی علیرضا زبرجدی علی مصطفایی هادی آتشی کیوان خانی Aassociation of growth hormone promoter region with twining rate in Markhoz breed goats Growth hormone gene Markhoz goat Odds ratio PCR-SSCP Twining 2016 1 01 329 336 http://mg.genetics.ir/article-1-1367-en.pdf
142-1368 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Investigating the genetic variation in the collection germplasm Sistan grapevine cultivars using IRAP marker سمیه خون‌رز حسین کمال‌الدینی مسیح فروتن براتعلی فاخری To study the genetic diversity of Sistan grape cultivars, 33 grapes varieties (Vitis vinifera L) of Zahak Research Station were evaluated by 10 retrotransposons based primers from ‘Ty1-copia’ and ‘Ty3-gypsy’ families (IRAP). The observed polymorphism for any markers Vine1Fb, Gret1F.Rb, Gret1F.Ra and Gret1Rb were 54.5, 83.0, 85.7 and 85.7%, respectively. The results, revealed this probability that the Gret1 retrotransposon in evolution process of these plants has been more translocated and amplified the number of additional copies within the genome. Cluster analysis based on Jaccard's similarity coephicient and UPGMA algorithm was divided the population into two groups at 0.24 similarity coephicient; one group with ten sub-groups and one group with two varieties. The most number of effective allele and the most diversity were related to VineF.b primer with 0.6565 Shannon’s index and the least number of effective allele and the least diversity were related to Gret1R.a and VineF.R.b primers with 0.4226 Shannon’s index, respectively. The results of principal coordinate analysis revealed the results of cluster analysis and extracted six coordinates that explained 45.5% of the total variation of the studied population. Diversity Grapevine IRAP Retrotransposon 2016 1 01 337 346 http://mg.genetics.ir/article-1-1368-en.pdf
142-1370 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Assessment of some gene transformation methods in microalgae Dunaliella salina محمدرضا صفرنژاد محمد علیمردانی عبدالقادر سعدی زاده مولود خلیلیان محمد حسین دانشور محمد علی ابراهیمی غلامرضا صالحی جوزانی Microalgae Dunaliella salina is a single-cell eukaryotic organism which is able to grow in high salt concentration. It has several advantages as a green bioreactor for producing of several pharmaceutical drugs including vaccines, hormones, growth factors, monoclonal antibodies and other recombinant proteins. In present study some gene transformation methods were applied for insertion of foreign genes into the microalgae cells. In the first step and to determine suitable selectable marker, some growth inhibitory compounds, such as kanamycin, hygromaicin, chloramphenicol and phosphinotricine were used. The results indicate the sensitivity of micro-algae D. salina to phosphinotricine in concentration of 7 µg/ml. Then, the gene bar, encoding phosphinotricine transferase, was introduced as selectable marker in transformation process. The methods applied in gene transformation include agitation with glass beads, electroporation and gene gun bombardment. The constructs containing the GUS gene applied for optimizing of the transformation process. To evaluate the effect of different promoters, the cauliflower mosaic virus 35S and maize ubiquitine promoters were used for expression of the GUS reporter gen. The expression of GUS reporter gene was evaluated by histochemical staining method. The results obtained here proved efficiency of the glass beads method for successful expression of foreign gene. In stable nuclear transformation methods, the transformed D. salina cells were cultured on media containing phosphinotricine selectable marker. These results proved capability of 35S promoter in transformation using glass beads method. The construct harbouring HBsAg gene was applied for gene transformation into microalga by using biolistic, glass beads agitation and agrobacterium co-cultivation. The transformed cells were moved into selective media. The presence of foreign gene in the transformed cells was analyzed by molecular approaches including PCR and RT-PCR. These results prove capability of biolistic and glass bead approaches for successful gene transformation into D. salina. Dunaliella salina Gene transformation Gus reporter gene Hepatitis B virus Microalgae 2016 1 01 347 358 http://mg.genetics.ir/article-1-1370-en.pdf
142-1371 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Detect of dwarf genes in commercial cultivars of bread wheat in Iran میلاد حیدری علی اکبر شاه نجات بوشهری سید علی پیغمبری ولی اله محمدی Wheat is the most important grain food in viewpoint of cultivation and production. Dwarfing is one of the important traits in plant breeding and has critical role in Green Revolution. In this study, the Dwarfing allelic compositions (Rht5, Rht8 and Rht9) were tested in 150 wheat cultivars and in order to recognition of Rht1 and Rht2, their response to Gibberellic acid (GA3) was analyzed. In studied cultivars, Rht9 has highest frequency (41.56%), and Rht5 has lowest frequency (27.92%). The presence of dwarfing alleles in foreign originated cultivars was more than Iranian cultivars. Generally, accumulation of dwarf alleles had been the main trends in Iranian wheat breeding program. In conclusion, our results can provide a genetic Catalogue for commercially varieties of wheat which can be used in improvement programs. Improvement of our understanding about these cultivars can helpful in parents and offspring’s selection. DNA Dwarf height Rht Gibberellin hormone Wheat 2016 1 01 359 366 http://mg.genetics.ir/article-1-1371-en.pdf
142-1372 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 The effect of salicylic acid on expression of NHX1 and SOS1 genes in root of wheat seedlings under salt stress فاطمه ملکی رضا فتوت محمدرضا عظیمی فرید شکاری زهرا السادات شبر Salicylic acid (SA) is an important signaling molecule involving in plant response to stresses. In the current research, different responses of two wheat (Triticum aestivum L.) cultivars to salinity (0, 0.5,1 and 1.5 g NaCl per Kg of soil) and seed priming with 1200 uM salicylic acid (SA) were studied. Salinity stress significantly reduced seedling dry weight in both genotypes (P≥ 0.01). Plants primed with salicylic acid under salt stress, 1.5 g NaCl per Kg of soil, produced greater biomass in the shoots and roots of wheat seedlings. Salt treatment increased the expression levels of NHX1 and SOS1 in roots of wheat seedlings. Expression levels of the genes were higher in the plants primed with SA as compared with water primed plants. The expression levels of NHX1 increased significantly after treatment with NaCl and salicylic acid. Our data suggests that different mechanisms, including Na+ avoidance or accumulation in cellular compartments, have been developed in salt-tolerant wheat cultivar. Therefore, it is possible that salicylic acid up-regulates the expression of genes SOS1 and NHX1 through the activation of protein Kinases in salt stress signaling pathways, and finally increases tolerance to salinity. NHX1 Salicylic acid Salinity SOS1 Wheat 2016 1 01 367 378 http://mg.genetics.ir/article-1-1372-en.pdf
142-1373 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Karyotypic study of six ecotypes of two species of yarrow; Achillea millefolium and Achillea santolina محمد ضابط فاطمه افشاری In this research a cytological study was performed on six ecotypes of two Achillea species growing in Iran including Achillea millefolium and Achillea santolina. The root apical meristem of ecotypes was used. After pretreatment, fixation, hydrolysis, staining and selecting the appropriate metaphase plate and photographing of samples with photomicroscope was determined the chromosome morphology. The results showed that the basic number of chromosome is x=9 in 6 Achillea ecotypes. Three ecotypes including Aligoodarz, Meshkinshahr and Ardabil were diploid with 2n=2x=18 and three ecotypes including Arak, Ilam and Estahban were tetraploid with 2n=4x=36. The different Karyotypic parameters including L (chromosome long- arm length), S (chromosome short-arm length) and T (chromosome total length) were measured. The results showed that the maximum range of S was 1.2 in Aligoodarz ecotype and minimum range of S was 0.2 in Ardabil ecotype. The maximum range of L was 2.7 in Aligoodarz ecotype and minimum range of L was 0.8 in Ilam and Arak ecotypes. The maximum range of T was 3.9 in Aligoodarz ecotype and minimum range of T was 1.2 in Arak ecotype. The maximum range of Huziwara index %F was 20.84 in Meshkinshahr ecotype and minimum range of %F was 7 in Estahban ecotype. The maximum range of %RL was 7.82 in Aligoodarz ecotype and minimum range of %RL was 1.57 in Ilam ecotype. The evaluation of karyotypic symmetry parameters on Stebbins and Levan methods including %S, DRL, %TF showed that the most symmetric ecotype was Arak and the most asymmetric ecotypes were Estahban and Aligoodarz. Therefore Arak ecotype is old ancient species and Estahban and Aligoodarz ecotypes are new and more development. Achillea sp. Chromosome Cytogenetic Karyotype Symmetry 2016 1 01 379 390 http://mg.genetics.ir/article-1-1373-en.pdf
142-1374 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Assessment of silicon content and expression of its transporters encoding genes in wheat under drought stress امین اله چلوی رضا فتوت افشین توکلی زهرا سادات شبر Drought is one of the major environmental stresses that affect plants in different ways, and its massive occurrence resulted in economic losses in agriculture. Investigating the expression of aquaporin proteins genes is a way to clarify the mechanism of plant response to drought stress at the molecular level. in order to determine silicon element concentration, trace, and the expression pattern of genes involved in silicon uptake in wheat, the values of Yavarous, Navid, Hamoun, and N-80-19 were cultivated in the greenhouse under normal and dry treatments as factor design based on a completely randomized design. In the greenhouse conditions and in four-leaf stage, drought treatment was applied by interrupting the irrigation. The results of variance analysis of the concentration of silicon element in experimental values of four-leaf stage showed that there is a significant difference between Navid values of drought treatment and control treatment, and there was no significant difference between other values. There was no significant difference in drought conditions. In the control conditions, the values showed significant difference. Tasi1 gene expression showed significant difference in Navid variety under drought and control treatments which indicate the drought impact on increased expression in Navid varieties. Tasi1 gene expression in other varieties under different treatments was not significant. Tasi2 detected gene expression in other varieties under drought and control treatments did not differ significantly. Drought RNA Silicon Transporter Wheat 2016 1 01 391 398 http://mg.genetics.ir/article-1-1374-en.pdf
142-1375 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Study of marR gene in ciprofloxacin resistant gyrA mutants ریحانه عبادی راضیه پوراحمد محمدرضا محزونیه Multiple antibiotic resistance and organic solvents tolerance have been attributed to overproduction of the AcrAB-TolC complex in E. coli. The expression of acrAB operon is controlled by transcriptional activators such as MarA. MarA is encoded by operon marRAB which its expression is normally maintained at low level by the MarR, autorepressor of mar operon. The aims of this research are isolation of multiple antibiotic resistant mutants from E. coli gyrA mutants and also localization of possible mutations in marOR. MICs for tetracycline and chloramphenicol in 10 gyrA mutants with different levels of resistance to ciprofloxacin were determined by sequential dilution method in liquid medium. Tolerance of different ratios of organic solvents, including hexane and cyclohexane in solid medium was determined. Presence of mutation was investigated by amplification of marOR region, including upstream of marR and marR itself with PCR and DNA sequencing. By using CLC software, obtained sequences were compared with that of control strain (MG1655) obtained from NCBI. Comparison of mean MICs showed that 3 out of 10 mutants were slightly more resistant to tetracycline than MG1655. Comparison of mean of colony count showed that 3 above mutants had better growth on hexane. Among three mutants, two possess a mutation in marOR. These three mutants were used to isolates clones with higher MICs for tetracycline. Mean of colony count showed that these clones gained better growth on hexane and also they did not acquire extra mutation in marOR region. It is concluded that generation of mutation in marOR is not enough by itself to produce the multidrug resistance phenotype and complete activation of AcrAB-TolC. AcrAB-TolC Multidrug resistance marRAB operon MarA MarR 2016 1 01 399 406 http://mg.genetics.ir/article-1-1375-en.pdf
142-1376 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Study the expression pattern of miR159 and miR171 under drought stress in peach, almond and GN فضیله اسماعیلی بهروز شیران ندا میرآخورلی حسین فلاحی Micro RNAs are a class of small RNAs which don't code any protein, and they act as a negative regulator of gene expression in eukaryotes. miR159 and miR171 are the most important microRNAs playing a role in plant development and response to stress. In this study miR159 and miR171 were detected in peach, almond and GN leaf tissues. Two levels of drought stress was considered. RNA was extracted before (control) and after (stressed) the stress treatments and the miRNA stem–loop reverse transcription was accomplished. Expression level differences upon drought stress measured with qRT-PCR. To construct miRNA and target gene networks, RESNET Plant database of Pathway Studio software 7 (Ariadne Genomics, ELSEVIER) was used. The results indicated that miR159 and miR171 responded to stress and they targeted MYB33 and SCL6, respectively. MYB33 and SCL6 are involved in plant growth and development. Attenuated growth is a characteristic feature of plants subjected to stress, to slow down the metabolism and divert the resources for adaptation. miR159 and miR171 are involved in regulating plant growth and development, due to the nature of most of their target genes and their expression significantly changed in response to drought stress. Almond CDNA Drought stress MicroRNA Peach Target gene 2016 1 01 407 416 http://mg.genetics.ir/article-1-1376-en.pdf
142-1377 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Retrotransposon insertional polymorphism and genetic diversity in cultivated and wild grape genotypes معصومه فرخ نیا بابک عبدالهی مندولکانی حامد دولتی بانه ایرج برنوسی In the current investigation, inter retrotransposon amplified polymorphism (IRAP) and retrotransposon microsatellite amplified polymorphism (REMAP) markers were used to study the insertional polymorphism of retrotransposon families Tvv1, Edel, Vine1, Gentil and Huben and genetic diversity in 67 grape (Vitis vinifera L.) cultivars and wild genotypes. Totally, 103 loci generated using 2 IRAP and 13 REMAP primers which 83 (80.58%) was polymorphic. The mean of Nei’s diversity index for primers ranged from 0 (Gentil-A7) to 0.38 (Tvv1-808), averaging 0.27 for the studied grape collection. The Dice similarity coefficients varied from 0.57 (H6 and Dastarchin) to 1 (Rasha-SeyahMamoli) with a mean value of 0.78. The maximum values of Nei’s diversity index (0.32), number of effective alleles (Ne=1.54) and Shannon’s information index (I=0.47) was for retrotransposon Tvv1, showing its high activity and insertional polymorphism in the studied grape collection. Cluster analysis using Dice similarity coefficients and UPGMA algorithm grouped cultivars in 6 main clusters and differentiated cultivated and wild grapes. The UPGMA classification of the studied cultivars was confirmed using model-based methods, implemented in software Structure 2.3.1. It was concluded that used retrotransposon families especially Tvv1 are transpositionally active and could make insertional polymorphism in grape genome; hence molecular markers developed based on this retrotransposon could be used in grapevine genotypes and cultivar identifications. Genetic diversity IRAP REMAP Retrotransposon Vitis vinifera L. 2016 1 01 417 428 http://mg.genetics.ir/article-1-1377-en.pdf
142-1378 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Detection of potato virus Y (PVY) strains using RT-PCR in potato fields in Golestan province سون آی بغدادی سعید نصراله نژاد فروه سادات مصطفوی نیشابوری محمدصفا بغدادی Potato virus Y one of the most common and destructive viruses found in potatoes (Solanum tuberosum L.), and has a worldwide distribution. Generally, PVY have three strains including PVYN، PVYC ، PVYO and two recombinant strains PVYNTN and PVYWilga. In order to determine the strains of the virus in the Golestan province samples were collected from potato fields. Samples were verified using an antiserum against PVY by indirect ELISA and posetive samples used in purvey of cDNA and RT-PCR test. Final PCR product was sequenced and comparison was made between these isolates and a number of other PVY strains available in GenBank. Three strains PVYN , PVYO and PVYWilga was reported first time in this province. In phylogenetic analysis showed that investigated strains were grouped into 4 groups. In this tree, Iranian isolate had most similarity with strains of Poland, Germany, Canada and Brazil. PVYN with 64.03 percent distribution as the dominant strain has spread in potato fields in Golestan province. Coat protein Large nuclear inclusion protein Phylogenetic analyses Potato virus Y Strain 2016 1 01 429 436 http://mg.genetics.ir/article-1-1378-en.pdf
142-1379 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Genetic affinity of Betula pendula from Iran using trnH-psbA chloroplast marker حمید بینا حامد یوسف زاده محمد اسماعیل پور امید اسماعیل زاده Birch is one of the oldest remaining tree species in the Hyrcanian forest which categorized in plants with limited distribution, rare and critically endangered. Hybridization and polyploidy are two main reasons for uncertainty in taxonomy of the genus Betula in the world. The taxonomic status and genetic affinity of Iranian population of Betula with other species from this genus were investigated. Leaf samples were collected from four Birch populations form North and North west of Iran and after DNA extraction, trnH-psbA region amplified. Comparative sequence analysis of PCR product from five populations in iran with 17 sequences of other species of the genus Betula recorded in NCBI were done. Phylogenetic analysis indicated that birches were in three clades A, B and C which all Iranian samples located in clade A. Sharestanak population formed the subclade Aa, whereas the Siahmarzkouh, Sangedeh, Nohshar and Marmisho comprised subclade Ab. Based on ABGD method, the birches of Iran comprised three separated groups. Sharestanak sample showed the greatest similarity with Betula pendula based on K2P methods. Finally, this study support the belong of Iranian populations to subgenus Betula. DNA barcoding Haplotype diversity Phylogeny Polyploidy trnH-psbA 2016 1 01 437 446 http://mg.genetics.ir/article-1-1379-en.pdf
142-1380 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Induced mutation in gene controlling ice nucleation activity by EMS, in two strains of Pseudomonas viridiflava like bacteria سیده فاطمه رشیدایی حشمت اله رحیمیان غلامعلی رنجبر Ice nucleation active bacteria in high populations are the cause of frost injury to the plants at higher subzero temperatures. Ice nucleation genetic base of bacteria is single chromosomal gene ina. To inducing mutations in ina gene, Pseudomonas viridiflava like bacteria were incubated with the mutagenic material (EMS) in 20 and 25 µl/ml concentrations.To track ina gene in isolates of P. viridiflava like polymerase chain reaction was used by ina gene special primer that was designed of conserved regions of gene in several species of ice nucleation bacteria. Ice nucleation activity was assessed using a drop of freezing. To investigate the loss of ice nucleation activity in suspensions containing 107 cells per ml (0.5 absorbance units at 600 nm) of each mutant was sprayed on orange seedlings and seedlings treated with sterile distilled water were used as negative controls. Seedlings were kept for 7 to 10 hours at -10 °C and then were returned to the greenhouse. Four mutant colonies were not able to create any frost damage on experimental seedlings. EMS Ice nucleation Mutant Pseudomonas viridiflava 2016 1 01 447 450 http://mg.genetics.ir/article-1-1380-en.pdf
142-1381 2024-03-29 10.1002
فصلنامه علمی ژنتیک نوین MGj 2008-4439 2008-4439 2016 10 3 Effects of cypermethrin on DNA breakage in the liver of Zebrafish (Aphanius sophiae) under different nutritional status مریم نصراله پورمقدم هادی پورباقر سهیل ایگدری Cypermethrin is one of the most effective pesticides that gets into aquatic ecosystems. At the present study, effects of frequency of feeding on toxicity of cypermethrin were investigated in Zebrafish (Aphanius sophiae). The specimens were exposed to different treatments for 14 days, i.e. (1) the absence of poison and feeding every three days in the morning, (2) the absence of poison and feeding every day (morning and evening), (3) the presence of poison and feeding every three days in the morning and (4) the presence of poison and feeding every day in the morning and evening. Then the liver was sampled and its DNA was extracted. The DNA was run on a electrophoresis gel and the percentage of breakage was calculated using fuzzy logic contol method. The results showed a highly significant effect of feeding frequency and poison on DNA breakage. Aphanius sophiae Cypermethrin DNA breakage Fuzzy logic 2016 1 01 451 454 http://mg.genetics.ir/article-1-1381-en.pdf