2024-03-29T13:12:20+03:30
http://mg.genetics.ir/browse.php?mag_id=143&slc_lang=fa&sid=1
143-1382
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Effect of cross breeding on the tolerance of Chinese based silkworms (Bombyx mori L.) to nuclear polyhedrosis virus
سیدهآزاده جوادیتکلیمی
ساحره جوزیشکالگورابی
رامین صیقلانی
he nuclear polyhedrosis virus (NPV), producing grasserie disease in silkworm, is accounted as one of the most important reason for production loss in sericulture industry. Cross breeding is one of the most important and effective breeding methods which could improve silkworm performance. In the current study, the resistance of larvae to NPV in 104 and 154 Chinese based lines and their hybrids in F1 and F2 generation were investigated. Fourth instar larvae were inoculated by NPV and their mortality were checked. A great value of heterosis and heterobeltiosis was observed in F1 hybrids, due to the susceptibility of line 104 and the tolerance of line 154 to NPV. The value of heterosis and heterobeltosis in reciprocal crosses varied from 82.91 to 86.19 and 27.26 to 29.54, respectively. Reciprocal crosses, however, did not declared significant difference in the susceptibility of larvae to NPV. Heterosis was declined in F2 and maternal heterosis was completely expressed on this generation (13.80 to 17.89 percent). Reciprocal cross did not have considerable effect on the maternal heterosis which indicates that the use of tolerant line as maternal line or paternal line do not make considerable difference.
Crossbreeding
Silkworm
Tolerance
Nuclear polyhedrosis virus
2016
2
01
455
460
http://mg.genetics.ir/article-1-1382-en.pdf
143-1383
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Construction and transformation of cassettes containing human Proinsulin gene for transient expression in alfalfa plants
فرگل مظاهری
بدرالدین طباطبایی
اسماعیل قاسمی
منصور امیدی
سیروس قبادی
هوشنگ علیزاده
urrently, millions of people all over the world for escaping the lethal effects of diabetes require persistent injections of insulin. Thus, to develop a system with low cost and high production of insulin that can fit into the consumers, is essential. With evolving genetic engineering techniques, production of human insulin in bacteria and yeasts were feasible, but due to drawbacks of producing recombinant proteins in those organisms including high cost, the possibility of contamination with toxic proteins and costly purification steps, most research has focused on the production of recombinant proteins in plants. Therefore, this investigation was done to express transiently the human proinsulin in alfalfa leaves by two different constructs. Construct pLPC as a fusion gene B subunit heat liable toxin of E.coli - proinsulin - penetratin and constructs pEPC incorporation into a peptide containing extensin leader - Proinsulin - penetratin. These constructs were transferred into Agrobacterium strain LBA4404 and agroinfiltration. Gene expression in alfalfa leaves was attested by RT-PCR and specific primers. The production of active proinsulin was confirmed by medical diagnostic mono-bind kits. The amount of production of active proinsulin in agroinfiltrated alfalfa leaves with constructs pLPC and pEPC were estimated 76 and 71.6 nano-grams per gram of fresh alfalfa leaves respectively.
Agroinfiltration
Extensin
E. coli
Penetratin
Subunit heat liable toxin
2016
2
01
461
468
http://mg.genetics.ir/article-1-1383-en.pdf
143-1384
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Analysis of quantitative trait loci affecting growth traits of Kermani sheep on chromosomes 1, 3 and 6
محیا حمیدی راوری
علی اسماعیلیزادهکشکوئیه
محمدرضا محمدآبادی
احمد آیتاللهیمهرجردی
he population studied hare was consisted 267 lambs from six half-sib families. each family contained 40 to 50 lambs. The genotype of all six male parent and their progeney determined for 243 microsatellite on chromosome. Growth on traits (birth weight, 3 month weight, 6 month weight and 9 month weight) recorded on all of progeny. The interval mapping method based on the least squares regression was used for analysis of gene position related to quantitative trait loci (QTL).The QTL of birth weight, 3 month weight, 6 month weight traits on chromosome 1 located on regions 30-34, 90-91 and 68-71 cM to centromere. The closest markers to QTL were MCM13, MAF4, and DIK5034, respectively. Gene position of QTL affective on birth weight on chromosome 3 was located on 26 cM and near marker was OARVH130. QTL related with 6 month weight on this chromosome was located on 69 cM to centromere and vicinal to marker MNS37A. The results showed the controlling QTL of birth weight on chromosome 6 was located on position 58-62 cM to centromere. Chromosome 1 had maximum and chromosome 6 had minimum QTL for traits checked and every three chromosome had birth weight control genome position but significant QTL for 9 month weight was not identify on any chromosome.
QTL Analysis
Quantitative trait loci
Kermani sheep
Microsatellite markers
2016
2
01
469
476
http://mg.genetics.ir/article-1-1384-en.pdf
143-1385
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Isolation, identification and increasing of lipid production by using UV radiation and replica-printing method in Rhodotorula musilaginosa
مرجان انشاییه
محبوبه مدنی
آزاده عبدلی
ایرج نحوی
pplication of microbial lipid as the superseding source of oil for human utilization was regarded in the first years of 20th century. One of the important aspects of single cell oil production is producing of microbial lipid rich in unsaturated fatty acids. Another importance of microbial oil is its application in biodiesel production, so isolation of oleaginous yeast with high potential of lipid production and efforts for increasing lipid content of them has great importance. In this investigation the goal was increasing lipid production of Rhodotorula musilaginosa using UV mutation technique. For this purpose at first oleaginous yeast Rhodotorula musilaginosa was isolated. Produced oil was analyzed by FTIR spectroscopy and gas chromatography - mass spectrometry (GC-MS) technique. The strain was identified by PCR method, after that the effect of UV mutation on its lipid yield was evaluated. For screening of mutated colonies with higher potential of lipid production, replica-printing technique was used. During different mutations one of the screened colonies with this technique had increasing in lipid content from 33.7% to 58.62% which was equal to 3.05 g/l increasing in lipid yield. These results show that there are valuable native strains with potential of increasing of lipid production using mutation and can be used in different industrial fields such as biodiesel production.
Mutation
Oleaginous yeast
Microbial lipid
Rhodotorula musilaginosa
2016
2
01
477
486
http://mg.genetics.ir/article-1-1385-en.pdf
143-1386
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Identification of molecular markers linked to chromosomal regions involving in seed Zn content in barley (Hordeum vulgare L.)
بابک عبدالهی مندولکانی
میرحسن رسولی صدقیانی
بهزاد صادقزاده
ابراهیم سپهر
مراد جعفری
n deficiency in plants is one of the prevalent micronutrient deficiencies which reduces both yield and nutrition values of the crops. In recent decades, considerable genetic diversity has been reported for tolerance to Zn deficiency in cereals and many efforts have being done to explore such a diversity in breeding programs. Nowadays, effective and reliable selection in plant germ plasms for quantitative traits has been possible using molecular markers and QTL analysis. Genetic maps with high density markers could play a significant role for efficient use of marker assisted selection (MAS) in breeding programs. In this study, IRAP, REMAP and ISSR markers were applied to saturate barley genetic map in a population of 150 doubled haploid individuals derived from a cross of Clipper and Sahara, and chromosomal regions involving in seed Zn content were also identified. The preliminary genetic map of this population was used as a frame for further analysis. Eighteen markers segregated in population, out of which 12 assigned to 7 barley linkage groups. Two markers deviated from the Mendelian ratio of 1:1 (P≤0.05). In the next step of this investigation, QTL analysis for seed Zn content was performed using 100 double haploids and 4 QTLs on linkage groups 2, 3 and 5 were identified. QTLs located on linkage groups 2 and 3 explained 0.09 and 0.11% of the total phenotypic variation, respectively. QTLs identified on linkage group 5 explained 0.13 and 0.12% of the total phenotypic variation of the trait. The results showed that retrotransposon markers can be effectively used for saturation of intervals with low marker density in barley genetic maps with low density. Also, the results could be a starting point for use of MAS in barley breeding programs for producing vanities with high seed Zn content and cloning of the genes involving in this trait.
Barley
DNA
Genetic map
Seed Zn content
QTL
2016
2
01
487
496
http://mg.genetics.ir/article-1-1386-en.pdf
143-1387
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Increasing of flavone synthase gene expression and flavonoid compounds and antioxidant enzymes activity of Cuminum cyminum by salicylic acid
طیبه فیروزی
صدیقه اسمعیلزادهبهابادی
براتعلی فاخری
لیلا فهمیده
uminum cyminum is a drug plant of Apiaceae and is full of secondary metabolite such as flavonoids and anthocyanin compounds. Salicylic acid belongs to a group of phenolic compounds acts as a key secondary messenger in activating specific defensive response of plant and also is used as inducer in provement of biosynthesis of secondary metabolites. In this research the effect of salicylic acid on gene expression of flavone synthase and flavonoid and anthocyanins compounds was studied. In addition the effect of salicylic acid on malondialdehyde , hydrogen peroxide and antioxidant enzymes activity of catalase, peroxidase and superoxid dismutase are studied. Seedlings of cuminum cyminum in vegetative growth stage (21 days) were treated by 1.5 mM salicylic acid and was harvested on 1, 2, 3 and 4 days after treatment. The results showed that gene expression of flavone synthase, flavonoid and anthocyanin compounds in comparison with the control increased significantly. The most increase was observed in third day which were about twice more than control. But in fourth day was decreased but still was greater than control. In fact according to the research the positive correlation between gene expression of flavone synthase and flavonoids and anthosyanins compounds was observed. Comparing with control, the results showed that malondialdehyde, hydrogen peroxide and antioxidant enzymes activity are increasedin different harvest stages under treatment with salicylic acid. So salicylic acid as an inducer increased antioxidant system of plant and increased flavonoid and anthocyanin compounds of cuminum cyminum by increase of flavone synthase gene expression.
Cuminum cyminum
Elicitor
Flavone synthase
Secondary metabolite
2016
2
01
497
506
http://mg.genetics.ir/article-1-1387-en.pdf
143-1388
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Identification of the Fol-SIX1 gene homolog in the genome of Fusarium oxysporium f. sp. melonis
فرهاد شکوهیفر
الهه ربیعی مطلق
ecreted-in-xylem1 (SIX1) secreted by Fusarium oxysporum f. sp. Lycopersici (Fol) during infection of tomato plants and plays a critical role as Avr (avirulence) gene in the interaction between the Fol and Tomato lines harboring I-3 resistant gene. SIX1 homologues genes are reported from some of F. oxysporum forma especials but steel presence of SIX1 in F. oxysporum f. sp. melonis (Fom) is unknown. Fom which infects melon cultivars causes important yield losses in melon (Cucumis melo L.) crops worldwide including Iran and specially Khorasan area. Identification of the effector genes in Fom could facilitate breeding procedures of resistant melon lines. In this study, to finding the SIX1 homolog in Fom, we initially aimed bioinformatics based approaches. The Fol-SIX1 protein sequence searched in the WGS data bank of Fom using tBLASTn tools with E-value cut off of 10. Four contigs have been identified that only one of them showed good coverage and similarity with Fol-SIX1. Sequence of the contig was extracted and putative ORFs have been predicted using vector NTi. ORF lined on antisense strand codes a protein with 283 amino acids and showed highest similarity with Fol-SIX1. Prediction of the gene structure preformed using FGENESH tools. In order to detect Fom- SIX1 in two isolate of Fom, we used a pair of internal specific primers. The entire nucleotide sequence of the gene was determined by a pair of external primers and compared with homologous sequences. In this study, we identified for the first time, homolog of Fol-SIX1 in the genome of Fom. We named this putative gene Fom-SIX1. This new gene encodes a protein harboring a signal peptide, eight cysteine residues and four disulfide bond connectivity which could make it considerable as a putative effector.
Homolog gene
Fol-SIX1
Fusarium oxysporum f. sp. melonis
Secreted protein
2016
2
01
507
578
http://mg.genetics.ir/article-1-1388-en.pdf
143-1389
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Study of genetic diversity and response of Artemisia species to salt stress
مریم ریاحی
حسین دشتی
اصغر رحیمی
الهام دهقان
rtemisia is the most important bush plants in natural plant communities. This plant i due to essential medicine and aromatic compounds have economic value. In this study, two experiments were conducted. In first experiment, genetic diversity 19 genotype of four species Artemisia were evaluated using 15 RAPD primers. Primers mplified 108 locus and produced a total 998 bands. Primer "F" has the highest genetic diversity index (0.58). Genetic similarity was calculated by employing Dice index and cluster analysis was carried out according Complete linkage algorithm that showed a similarity range (0.4-0.8).The highest similarity between the two samples A.sieberi (nodoshan) and A.khorassanica-678 (0.80). Cluster analysis, classified genotypes into 3 main groups at 0.46 coefficient of similarity. There was little relationship between genetic divergence and geographical origins. The results indicated the existence of genetic variation among Artemisia genotypes and high efficiency of RAPD markers in the expression of genetic variation between species and within species of Artemisia. In second experiment, the germination characteristics of five genotypes from two species were studied in salinity stress. A factorial experiment was conducted with two factor; salinity (0, 3, 6 and 9 dSm-1) and five genotypes .The germination percent, germination rate and mean of germination time were measured. The result showed that germination percent and germination rate were reduced by increasing salinity. Species A. sieberi (nodoshan) has the highest germination percent and was the most tolerant accession.
Artemisia
Genetic diversity
RAPD
Germination
Salinity
2016
2
01
519
529
http://mg.genetics.ir/article-1-1389-en.pdf
143-1398
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
QTLs analysis of physiological traits in the Steptoe/Morex population of barley grown under saline and normal environments
براتعلی فاخری
حبیبه شهرکی
The salinity stress after drought is the most important and the most common environmental stresses that limit crop production worldwide, including Iran. In order to determine the genomic regions of some physiological traits of "steptoe×morex" drived doubled haploid population of barley under normal and salinity stress conditions, an experiment was conducted at Research Farm, The experiment arranged in two randomized complete block design with two replication. Each plot included four rows with length of 3 m and 25 cm inter-rows distance. Triats such as chlorophyll content, chlorophyll fluorescence (Fo, Fv and Fv/Fm), relative water content (RWC), prolin content, water soluble carbohydrate (WSC) and cell membrane stability (CMS) were measured. QTL analysis was carried out using genetic linkage map derived from 327 molecular marker of RFLP and QTL cartographer software with composite interval mapping method. Combined analysis of varianc showed significant difference between the lines for all the studied traits. The maximum correlation was observed between Fo and Fv/Fm. In general, we found 43 QTLs (13, 16 and 14 QTLs for normal, salinity stress and mean of two conditions, respectively.) for the studied traits. Phenotypic variances that were explained by these QTLs changed from 9.06 to 30.28, respectively, for Fo (qFO 1m) and Fv/Fm (qFvFm 2.1m) in the mean of two conditions. LOD scores were ranged from 2.61 to 8.74, respectively, for the QTLs of chlorophyll content (qCHC1m) and Fv/Fm (qFvFm 2.1m) in mean of two conditions. QTL position 81.2 cM of chromosome 2H, controlling Fv/Fm, was quite stable. Therefore, it can be used in marker asisted selection.
Barley
Physiological traits
QTL
Salinity stress
2016
2
01
531
547
http://mg.genetics.ir/article-1-1398-en.pdf
143-1399
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
To develop a SCAR marker linked to rhizomania resistance gene in sugar beet
پیمان نوروزی
Rhizomania is the most important disease of sugar beet in Iran and some other parts of the world, and plays an essential role in decreasing sugar yield in fields. The best approach to control this disease is to use resistant varieties. For the involvement of resistance genes in breeding programs, tagging these genes by molecular markers is necessary. The aim of this research was cloning of a RAPD marker linked to rhizomania resistance genes (Rz1) and convertingto SCAR specific marker. For this, at first the DNAs were extracted from the plants and then RAPD-PCR reactions done using the primer related to the marker. The RAPD products were excised from the gel, purified and cloned into plasmid vector. Then, the recombinant clones were selected and sequenced. The sequenced fragments were used to design specific primers by Oligo 5 software. Analysis of the specific primers in PCR reactions were produced specific single band for the marker linked to Rz1 gene. This SCAR marker was named to ZN1 and it has more specificity and reproducibility as compared with the original RAPD marker. Polymorphism of this marker was verified in different sugar beet genotypes. Therefore, this SCAR marker can be utilized in breeding programs of marker assisted selection (MAS) in sugar beet for selecting rhizomania resistant plants.
RAPD marker
Resistance
Rhizomania
SCAR marker
Sugar beet
2016
2
01
549
556
http://mg.genetics.ir/article-1-1399-en.pdf
143-1400
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Exploring the EST libraries for developing microsatellite markers in Aeluropus littoralis
مریم میدانسری
قربانعلی نعمت زاده
احسان شکری
EST sequences recorded in the Gene bank database are an important source for microsatellite discovery in coding genome. These sequences give us the opportunity to discover new genes, as well as a resource for development of markers. Unlike genomic microsatellite markers (non-coding), EST-based microsatelite markers (EST-SSR) are effectively extracted from free publicly EST libraries with less time and expense and show the good performance. In this research using the halophyte grass, Aeluropus littoralis EST library and insilico analysis microsatelite motifs were identified and based on that 16 polymorphic markers (ALES), was developed for genomic studies. The results showed that in this plant (6.7%), such as rice (4.7%), there is a high rate of microsatellite in the coding regions. Also, tg, (gcg- gaa) and ttga motifs constitute the major variety of SSR motifs in a set of Aeluropus EST records. The polymorphism information content (PIC) values ranged from 0.27 to 0.37 with an average of 0.32 that higher values goes to ALES number 3,1,11,13 and 15. An average similarity coefficient (Jaccard) between samples was reached to 55%. These results suggest a great genetic variability in genomic coding regions of A. littoralis plant. Finally, cluster analysis grouped the A. littoralis ecotypes into six groups.
Aeluropus littoralis
EST-SSR
In silico analysis
Microsatelite
2016
2
01
557
566
http://mg.genetics.ir/article-1-1400-en.pdf
143-1401
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Study of different sulfur concentrations on Alliinase gene expression profile in some garlic (Allium sativum L.) clones with QPCR
علی عمارلو
کمال کاظمی تبار
حمید نجفی زرینی
Apot culture experiment was carried out to assess the effect of 6 sulfur concentrations (0, 6, 12, 18, 24 and 60 g) on alliinase gene expression in 3 Iranian garlic clones on randomized block designed in 3 replications. Results based on Real-time quantitative PCR indicated the responses of garlic cultivars could be reflected as changes in gene transcripts but all gene expression patterns revealed no positive and continuously correlation. Gene expression decreased in sulfur supply conditions (6 to 18 g) firstly and then (18 to 24 g) increased intensively. At very high concentration (60 g) restate it reaches its minimum value. The findings of this research can be used in further research on the garlic metabolic engineering of active ingredients and also reducing or enhancing the activity of the responsible genes for garlic pungency.
Alliinase
cDNA
Garlic
Organosulfur compounds
Pungency
2016
2
01
567
573
http://mg.genetics.ir/article-1-1401-en.pdf
143-1402
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Evaluation of genetic variability in Iranian Thymus daenensis subsp. daenensis, by use of inter simple sequence repeat (ISSR) markers
بهنام مندک
ولی اله محمدی
حسن زینالی
جواد هادیان
Thymus is one of most important and valuable plants in the world. In order to investigation of genetical diversity of Thyme genus، 13 population of Iranian Thymus deanensis , planted in green house and DNA from young leaves was extracted. In order to genetical diversity analysis, 12 ISSR primers were used. Among used primers, 10 primers produced unique band and 57 allel were detected. The size range of the amplified products was 200–2000 bp. Based on Issr data cluster analysis, studied populations classified in two independent groups. The population genetic distance were different from 0.041 for lorestan and kordestan to 0.282 for lorestan and Isfahan. evaluation of genetic diversity within populations by Nei genetic diversity analysis and shanon information index indicated that the diversity within Daran Isfahan population (I=0.43, h= 0.29) was more than other populations while the genetic diversity within Semirom population (I=0.2 , h= 0.13) was less than other populations. The intra and inter populations diversity was 75% and 25%, respectively. In this research, the primers that had AC and AG motifs produced clearer bands in Th. daenensis than those with other motifs. Cross between Isfahan and lorestan population that have a distance to gether can have an important role to get a good population. Thymus deanensis have a high genetic variety that could be considered in hybridization and breeding.
Thymus
Genetic diversity
ISSR molecular marker
Medical plants
2016
2
01
575
584
http://mg.genetics.ir/article-1-1402-en.pdf
143-1403
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Change in cysteine synthase gene function in Arabidopsis old3 mutant plants in response to oxidative stress-induced by cadmium chloride
فرناز نوروزی
رضا شیرزادیان خرم آباد
محمود قاسم نژاد
عاطفه صبوری
پیمان منبری
Arabidopsis mutant old3 (Onset of Leaf Death3) has a EMS mutation in Cysteine synthase gene(AT4G14880). One of the important functions of this gene is plant detoxification against oxidative stress. Old3 plants exposed to cadmium go through plant cell death after 12 days at 22˚C. Since the growth and development of old3 plants return to a normal situation at 28˚C, this research was performed to evaluate old3 plant responses to cadmium at 28˚C and 22˚C and compared with the wild type plants, Landsberg erecta (Ler-0). Therefore several traits including germination rates, growth rates and fresh weights were studied. POD and CAT enzyme activities were assayed in mutant seedlings. In addition, the relative expression levels of two marker genes, DEFL and GST1, which are involved in oxidative stress was measured. Factorial statistical design based on a completely randomized design was used to analyze the data. The plants growth, fresh weight and germination rate under the cadmium stress at 28˚C were higher than those under 22˚C, and it happened more clearly in the mutant plants. The result suggested that the resistance of wild type was higher at both two 28˚C and 22˚C. The investigation of POD enzyme activity at 28˚C indicated higher activity in the mutant plants compare to wild type. Furthermore, the relative expression levels of DEFL and GST1 genes in the mutant old3 plants were enormously higher than those of wild type at 28˚C. As a result, it was found that although the growth and development of old3 plants could be recovered by the temperatures, but the mutant old3 plants were more sensitive to the cadmium contamination compared to the wild type Ler-0 plants at both temperature and could not be recovered into the wild type responses at 28˚C.
Arabidopsis
Cadmium chlorid
Oxidaitive stress
old3 point mutation.
2016
2
01
585
596
http://mg.genetics.ir/article-1-1403-en.pdf
143-1404
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Investigation of mitochondrial DNA polymerase gama (POLG) in patients with multiple sclerosis
حسین سلطان زاده
محمد حسین صنعتی
میترا عطایی
مسعود هوشمند
سید مسعود نبوی
کاظم پریور
Multiple sclerosis (MS) is an autoimmune multifactorial disease that usually develops in susceptible young adults. A possible involvement of mitochondria in MS has been postulated because of a higher rate transmission of the disease from mother to child than from father to child. Also association between Leber’s Hereditary Optic Neuropathy, a mitochondrial disease and MS is another evidence. Fatigue is a common problem of the MS patients which is related to the energy production difficulty by the mitochondria. To investigate further the relationship between MS and mitochondria we analysed the gene encoding for polymerase G (POLG). Mutations in this gene are related to 50 different diseases. Total genomic DNA was extracted from 61 idiopathic MS patients and 40 controls. Primers were designed to amplify the 4 hot exones 7, 8, 9 and 13 and following by sequencing. The analysis of sequences showed no difference between control and patient samples. However this does not exclude the association of the gene in MS but it needs more investigation. Also it is necessary to test the other exones of the gene.
DNA
Multiple sclerosis
Mutation
Polymerase G (POLG)
2016
2
01
597
600
http://mg.genetics.ir/article-1-1404-en.pdf
143-1405
2024-03-29
10.1002
فصلنامه علمی ژنتیک نوین
MGj
2008-4439
2008-4439
2016
10
4
Bioinformatic analysis and structural modeling study on DNA repair helicase (UvrD) from Arabidopsis thaliana and Oryza sativa
افسانه سادات فرساد
سعید ملک زاده شفارودی
Mismatch repair (MMR) proteins play important roles in maintaining genome stability in all the organisms. UvrD helicase is a component of MMR complex and plays an essential role in the DNA repair by providing the unwinding function. In the current manuscript we presented a bioinformatic analysis of UvrD helicase from two plant species (Arabidopsis and rice). The Arabidopsis thaliana and Oryza sativa UvrD had 1149 (129 kDa) and 1165 amino-acids (130 kDa) proteins, respectively. These proteins were larger than the E. coli UvrD because they contained a longer N-terminal extension and linker sequences. This research showed UvrD in both plants contained ATP-binding site. The existence of the N terminal ATP-binding site and conserved motifs in both plants suggest that the N terminal and C terminal sequences can show ATPase and a 3' to 5' DNA helicase activity similar to the E. coli UvrD. To gain some insight into the structure of UvrD using bioinformatic analysis softwares, three- dimentional structure of this enzyme was predicted in two different plant species. Molecular graphic images and structural analysis of the best models were produced using the UCSF Chimera package. The results demonstrated that there were not obvious differences in three- dimentional structure of AtUvrd and OsUvrd and during the evolutionary changes they have preserved their major domains and biochemical characteristics like ATPase and DNA helicase activity.
Arabidopsis
DNA repair
Genotoxic stress
Helicase
Rice
2016
2
01
601
605
http://mg.genetics.ir/article-1-1405-en.pdf