Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

---

The roots and stolons of Glycyrrhiza species constitute one of the most important crude drugs in the world and contain a large amount of glycyrrhizin, an oleanane-type triterpenoid saponin. In this study, relative expression of the genes involved in the biosynthesis pathway of this secondary metabolite, including bAS, SQS, CYP88D6 and CYP72A154, was investigated in the presence of methyl jasmonate in a culture of cell suspension of licorice. Methyl jasmonate was added at a concentration of 100 μM in a stationary growth phase to cell suspension.  The control experiment had no methyl jasmonate. The cells were harvested at intervals 24, 48 and 72 hours’. The experimental was done as split plot in time based on completely randomized design with two replications. The results showed that the two bAS and CYP88D6 genes had the highest expression levels in 48 h after the treatment. SQS and CYP72A154 genes, had the highest expression levels in 72 hours after treatment, but the expression of CYP72A154 didn’t show significant difference comparing to control. The bAS and SQS genes showed higher expression than CYP88D6 and CYP72A154 genes, Because the bAS and SQS genes are involved in the initial pathway of biosynthesis and also are involved in the biosynthesis of other saponins, such as soyasaponin, while The CYP88D6 and CYP72A154 genes are involved in the final pathway biosynthesis for glycyrrhizin. The previous studies had been confirmed presence of glycyrrhizin in the root. In this study, the increased expression of the genes involved in the biosynthesis pathway of glycyrrhizin in cell suspension culture confirmed the presence of glycyrrhizin in cell cultures of licorice.
 

Keywords: licorice, gene expression, cell suspension, methyl jasmonate

Full-Text [PDF 360 kb]   (483 Downloads)    

Add your comments about this article : Your username or Email:

---