Volume 16, Issue 2 (7-2021)                   MGj 2021, 16(2): 143-150 | Back to browse issues page

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AHMADI LIVANI M, POURAKBAR L, SATEII A, EBADI M, mohamadi A. Evaluation of Germacrene A Synthase gene expression under salinity stress in Feverfew. MGj. 2021; 16 (2) :143-150
URL: http://mg.genetics.ir/article-1-1610-en.html
Abstract:   (339 Views)
Feverfew (Tanacetum parthenium) is an herbaceous plant belonging to the Asteraceae family, which is one of the valuable medicinal plants that its usage effect has been confirmed in preventing migraine remedy by reducing the swelling, pain and the spasm of the blood vessels. Parthenolide is one of the most important sesquiterpene lactone in feverfew, which contains more than 85% of the total sesquiterpene in this plant and is known as the effective material in this plant. The aim of the present study was to investigate the gene expression of Germacrene A Synthase Tp (GAS) in Feverfew plants under salinity stress and Parthenolide amount changes by HPLC and essence compound by GC/MS method. In the present study, young leaves of Feverfew were placed under different salinity levels (zero salinity EC=3.2, mild salinity EC=6.1, average salinity EC=8.4 and severe salinity EC=10.2). Additionally, the changes in the expression of Germacrene A Synthase Tp (GAS) was investigated using Real Time PCR. The content of produced Parthenolide in leaves was measured by HPLC extraction and essence percentage was measured using GC/MS instrument. The results demonstrated that there was a significant increase in the expression of the studied genes in the leaves of plants under salinity stress compared to the control plants (p≤0.01). Moreover, the highest expression of GAS gene was observed in severe and moderate salinity treatments. Significantly changes in gene expression was accordance with changes in the amount of produced Parthenolide in leaves. Moreover, the essence percentage under stress condition (0.66%) was more than control (0.49%).
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Type of Study: Applicable | Subject: Subject 01
Received: 2019/12/10 | Accepted: 2021/05/18 | Published: 2021/07/7

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