Volume 13, Issue 3 (12-2018)                   MGj 2018, 13(3): 333-341 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gholamian F, Talebi M, , Sayed-Tabatabaei, Sayed-Tabatabaei, Sayed-Tabatabaei, Sayed-Tabatabaei, Sayed-Tabatabaei, Sayed-Ta B, Shiran B. The Effect of Metyl Jasmonate Hormone on Gene Expression of Phenylalanine Ammonia Lyase in Arctium lappa L.. MGj 2018; 13 (3) :333-341
URL: http://mg.genetics.ir/article-1-70-en.html
Abstract:   (1572 Views)
The medicinal plant, Burdock (Arctium lappa L.), as a rich source of polyphenol antioxidant, is used to control and treatment of allergies, AIDS, cancer and diabetes type II. Phenylalanine ammonia lyase (PAL) is a key enzyme in the biosynthesis of polyphenol compounds in the phenylpropanoid pathway. To detect the PAL gene in the Arctium lappa, PCR reaction was performed using specific primers designed from conserved sequences of the gene in NCBI database. The sequence analysis (609 bp) revealed that the detected PAL gene is similar to those previously sequenced in the NCBI database. Methyl jasmonate is a cyklopentany compound of linolenic acid derivatives which can be produced through the octadecanoid pathway. There are evidences that methyl jasmonate as a signaling molecule interference in some signaling pathways that induces certain enzymes catalyze biosynthetic reactions for the formation of the defensive reaction and leading to the induction of immune responses. In the current study, gene expression of PAL gene was evaluated by applying of 50 and 100μm MeJA, at intervals of 24, 48 and 72 hours. Results showed that expression of PAL gene significantly increased in response to MeJA after 24h. The gene expression of PAL was significantly higher after 24h treating samples with 100μm MeJA compared to the control. The results of this study suggest that metyl jasmonate is an efficient abiotic elicitor which affect expression of PAL  invovled in  polyphenol compounds in Burdock.                 
Full-Text [PDF 548 kb]   (581 Downloads)    
Type of Study: Applicable | Subject: Subject 01
Received: 2016/10/15 | Accepted: 2018/10/7 | Published: 2019/10/2

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | فصلنامه علمی ژنتیک نوین

Designed & Developed by : Yektaweb