@ARTICLE{, author = {فهیمه خلجی, and بهروز شیران, and فریبا رفیعی, and }, title = {Effect of salt stress on expression pattern of miR160 and miR167 and their target genes in sunflower}, volume = {11}, number = {2}, abstract ={Salinity is a common environmental stress that seriously affects plant growth. The results of molecular researches in relation to environmental stresses showed that expression pattern of genes and their regulators were changed in response to stresses. miRNA's are an important class of gene expression regulators that diversely responds to environmental stresses and have a key role in post transcriptional regulation. In this study, the expression of miR160 and miR167 were studied by qRT-PCR at two salinity levels, (75 and 150 mM NaCl) and control condition in both root and leaf tissues. A factorial experiment based on completely randomized design(CRD) with three replication was used in this study. Plants were exposed to salinity stress in six-leaf stage and after 24 h sampling from leaf and root was performed then samples were freezed in liquid nitrogen. cDNA was synthesized with stem-loop RT-PCR procedure. Expression of miR160 and miR167 was measured using qRT-PCR technique. The results showed that the expression of miR160 was firstly increased and then decreased. While the values of fold change showed significant reduction of miR160 expression in 75mM salinity level, it didn’t show any significant differences comparing to control condition at 150 mM salinity. The expression of BU026935 gene in both salinity levels and tissues were increased in comparison to control. MiR167 was significantly down regulated at 75 mM salinity. The results of network analysis revealed that increasing miR167 expression under salinity condition is leading to auxin reduction. ARF6 expression level was decreased at 75mM salinity level in both root and leaf tissues. At 150 mM salinity level ARF6 gene showed down regulation only in root tissue. }, URL = {http://mg.genetics.ir/article-1-1420-en.html}, eprint = {http://mg.genetics.ir/article-1-1420-en.pdf}, journal = {Modern Genetics Journal}, doi = {}, year = {2016} }