RT - Journal Article T1 - Identification of polymorphism of G1 and G8 variants of GDF9 gene on multiple births Zel sheep JF - MGJ YR - 2016 JO - MGJ VO - 11 IS - 2 UR - http://mg.genetics.ir/article-1-1428-en.html SP - 307 EP - 312 K1 - GDF9 K1 - Polymorphism K1 - Prolificacy K1 - Sheep K1 - RFLP AB - The aim of this research was to investigate the polymorphism of prolificacy gene, GDF9 in Zel sheep breed using RFLP-PCR method and to study the association of genotypes with multiple birth records. Genomic DNA of 200 sheep was extracted using modified salting out extraction protocol to evaluate the mutations in G1 and G8 variants of GDF9 gene. Polymerase chain reaction was carried out to amplify a 462 and 139 bp fragments of GDF9 gene. The amplified fragments of G1 and G8 variants of GDF9 genes were digested with restriction enzymes Hin6I and DdeI, respectively. The G1 variant of GDF9 gene was polymorphic. Two mutant (B) and wild (A) type alleles with frequencies of 0.05 and 0.95 were detected in tested animals. Known's point mutation FecGH was monomorphic in tested animals. For G1 variant of GDF9 gene, a Chi-square test indicated that the population was in equilibrium. GLM procedure of SAS software was used to investigate the association of G1 variant of GDF9 gene with twining. Results indicated that there was no significant association (P>0.05) between polymorphism at this locus and twining. LA eng UL http://mg.genetics.ir/article-1-1428-en.html M3 ER -