fa مطالعه جهش‌های ژن B4GALNT2 (FecL) با روش تجزیه نقطه ذوب با وضوح بالا(HRM) در گوسفند زندی ژنتیک جانوری Subject 02 پژوهشي کوتاه Research <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="direction:rtl"><span style="unicode-bidi:embed"><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">از معیار‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">های مهم بررسی توان تولیدمثل، تعداد نتاج متولد شده در هر زایمان می</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">باشد. با توجه به تأثیر این صفت در سودمندی گله</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">ها</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">،</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> بهبود ژنتیکی آن به</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">کمک فناوری&shy;های مولکولی مورد توجه قرار گرفته است. تحقیقات نشان داده است که ژن</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">های عمده اثر شامل </span></span></span><span class="Heading1Char"><span style="font-weight:bold">GDF9, BMP15, ALK6</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> و </span></span></span><span class="Heading1Char"><span style="font-weight:bold">B4GALNT2</span></span><span class="Heading1Char"><span style="font-weight:bold"> (</span></span><span class="Heading1Char"><span style="font-weight:bold"><i>FecL</i></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">) با صفت تعداد نتاج در هر زایش مرتبط بوده</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">اند. تأثیر جهش ژن </span></span></span><span class="Heading1Char"><span style="font-weight:bold">FecL</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> در حالت هموزیگوت به</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">صورت افزایشی بوده و منجر به آزاد شدن سه تخمک برای دو کپی از این ژن می&shy;شود. هدف از این مطالعه بررسی چندشکلی </span></span></span><span class="Heading1Char"><span style="font-weight:bold">&nbsp;DLX3: c. * 803A>G</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">در شناسایی ژن </span></span></span><span class="Heading1Char"><span style="font-weight:bold">B4GALNT2</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> (</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><i>FecL</i></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">) روی کروروموزم 11 با استفاده از روش ذوب با وضوح بالا (</span></span></span><span class="Heading1Char"><span style="font-weight:bold">HRM</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">)</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">،</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> در گوسفند زندی و ارتباط این ژن با صفت چند&shy;قلوزایی بود. آنالیز </span></span></span><span class="Heading1Char"><span style="font-weight:bold">HRM</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> روشی است که میزان کاهش فلورسنت را در طی فرآیند شیب حرارتی ذوب </span></span></span><span class="Heading1Char"><span style="font-weight:bold">DNA</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> در اثر خروج رنگ اندازه</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">گیری می</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">نماید. نتایج حاصل از </span></span></span><span class="Heading1Char"><span style="font-weight:bold">HRM</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> با نتایج تعیین توالی در تطابق کامل بود. هیچ</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">گونه تغییری در توالی</span></span></span> <span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">نوکلئوتیدی</span></span></span> <span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">جایگاه مورد نظر در جمعیت گوسفند زندی مورد بررسی مشاهده نشد</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">،</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> از این رو مکان مورد بررسی برای ژن </span></span></span><span class="Heading1Char"><span style="font-weight:bold">B4GALNT2</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> یک شکل (مونومورف) می</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">باشد. ما در</span></span></span> <span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">تمامی توالی</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">های مورد نظر تغییرات نوکلئوتیدی به‌صورت حذف را مشاهده کردیم، اما تغییری در آمینواسید حاصل و در نتیجه تغییری در ساختار پروتئین حاصل نشد. به</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">علاوه دو جهش شامل جهش </span></span></span><span class="Heading1Char"><span style="font-weight:bold">A>G</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> در جایگاه 2242در نمونه 7 و جهش </span></span></span><span class="Heading1Char"><span style="font-weight:bold">C>G</span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA"> در جایگاه 2241 در نمونه 77 مشاهده شد. بررسی جهش در ژن</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">‌</span></span></span><span class="Heading1Char"><span style="font-weight:bold"><span lang="AR-SA">های عمده اثر دیگری برای این نژاد پیشنهاد می&shy;شود.</span></span><span style="font-weight:bold"></span></span></span></span><br> <span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA"><span style="line-height:150%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br> &nbsp;</div> <div dir="rtl" style="text-align: justify;">Litter size is one of the most important reproductive criteria in animals. Hence, the effect of this trait on the productivity of the flock has been addressed by its genetic enhancement through molecular technologies. Previous studies have shown that the major genes including GDF9, BMP15, ALK6, and B4GALNT2 (Fec^L) are associated with litter size. The effect of Fec^L mutation in homozygous states is additive and results in the release of three ovum for two copies of this gene. The aim of this study was to investigate polymorphism DLX3: c.&shy;* 803A> G in B4GALNT2 (Fec^L) gene on chromosome 11, using High Resolution Melting technique (HRM), and it is association with litter size in Zandi sheep. HRM analysis is a method that reduces the amount of fluorescence during the process of thermal melting of the DNA through color exhaust. The results of HRM were consistent with the sequencing results. No mutations were observed in the nucleotide sequence of the target studied in the Zandi sheep. Therefore, B4GALNT2 gene is monomorphic for this site length in 232bp fragment of this gene. We discovered many deletions, but there was no change in the resulting amino acid. Therefore, that did not result in structural alteration of the protein. In addition, two new mutations included A> G mutation at position 2242 in sample 7 and mutation C> G at position 2241 in sample 77 were detected. It seems necessary to investigate mutations in other major genes for this breed<br> &nbsp;</div> چندقلوزایی, تنوع ژنتیکی, نشانگر های ژنتیکی, HRM, ژنFecL litter size, genetic variation, genetic markers, FecL, HRM 87 90 http://mg.genetics.ir/browse.php?a_code=A-10-240-1&slc_lang=fa&sid=1 Abdolreza Salehi عبدالرضا صالحی arsalehi@ut.ac.ir 10031947532846006403 10031947532846006403 Yes University of Tehran دانشگاه تهران Farnoosh Rostami فرنوش رستمی farnooshrostami69@yahoo.com 10031947532846006404 10031947532846006404 No University of Tehran دانشگاه تهران Mohammad Reza Bakhtiari Zadeh محمد رضا بختیاری زاده mrbakhtiari@ut.ac.ir 10031947532846006405 10031947532846006405 No University of Tehran دانشگاه تهران