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Tomato is one of vegetables of Solanaceae family that consumes in different ways. Tomato breeding for heterosis and resistance/tolerance to drought is an important program started since 2014 at Ilam University, for which identification of more variable parent lines was the main objective breeders. In the current research, in order to evaluate the relationship between and genetic distance of lines and isolation of the best parents among 36 tomato lines (collected from surround the globe) for hybridization, ISSR molecular markers were used. PCR reactions were performed using 11 primers which produced an appropriate and distinguishable model for the 36 studied genotypes. Totally 89 alleles were identified on agarose gel. Total number of alleles per primer ranged from 7 to 10. The highest and lowest polymorphism recorded for marker ISSR17 with 90 percent and marker LBMB B with 71 percent, respectively. Maximum and minimum of PIC were 0.45 (LBMB C, LBMB D, HB12) and 0.38 (ISSR17). The highest marker index (MI) was 3.8 (LBMB A) and 3.51 (primer 809), indicated a higher resolution of the primers compared to the others. Average of marker index was 3. Dice genetic similarity coefficient ranged from 0.10 to 0.96. Results of the research revealed that genotypes SolanumPimpinellifolium, S. Chilense LA1959 and S. ChilenseLA1972 are the best choices for heterosis breeding and crossing with indigenous Iranian varieties.

Keywords: Molecular markers, Polymorphisms, Hybrid, Genetic distance.

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