Evaluation of proline biosynthetic pathway of chickpea through putrescine degradation under cold stress

Amini, Saeed; Maali-Amiri, Reza; Poormazaheri, Helen

Volume 17, Issue 3 (11-2022) MGj 2022, 17(3): 251-264 | Back to browse issues page

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Amini S, Maali-Amiri R, Poormazaheri H. Evaluation of proline biosynthetic pathway of chickpea through putrescine degradation under cold stress. MGj 2022; 17 (3) : 2
URL: http://mg.genetics.ir/article-1-1770-en.html

Evaluation of proline biosynthetic pathway of chickpea through putrescine degradation under cold stress

Saeed Amini

, Reza Maali-Amiri ^*

, Helen Poormazaheri

University of Tehran

Abstract: (1052 Views)

Metabolic acclimation to cold stress plays an important role in the survival, growth and yield of crops. Proline, as an osmolyte may take part in counteracting cold-induced oxidative stress in chickpea. In this experiment, content of hydrogen peroxide (H₂O₂), putrescine (Put), proline, activity of diamine oxidase (DAO) and relative expression of diamine oxidase (DAO) gene in cold-tolerant (Sel96th11439) and cold-sensitive (ILC533) chickpea (Cicer arietinum L.) genotypes under cold stress (4 °C) as a factorial experiment in a Completely Randomized Design were studied. In tolerant genotype, H₂O₂ content after a significant increase on the first day of cold stress decreased significantly on the sixth day of cold stress compared to control condition (up to 4.7%), while its accumulation was observed in sensitive genotype (up to 50%). These results indicated a relative acclimation to cold stress in tolerant genotype. Under cold stress, GABA and Put contents in tolerant genotype were higher compared to sensitive genotype (up to 1.47- and 3.2-fold, respectively). Under cold stress, increasing GABA content in tolerant genotype was accompanied with an increase in DAO activity and relative expression of DAO gene as biosynthetic pathway of this metabolite (up to 1.87- and 1.72-fold, respectively). The maximum activity of this route was observed in tolerant genotype on the sixth day of cold stress. Under cold stress, accumulation of proline in tolerant genotype led to reduce cell damage (H₂O₂ results) and improved cold tolerance. These indices were useful in assessment of chickpea genotypes under cold stress and breeding programs.

Article number: 2

Keywords: Hydrogen peroxide, Putrescine (Put), Proline, Diamine Oxidase (DAO), Relative expression of Diamine Oxidase (CaDAO) gene, Cold tolerance.

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Type of Study: Applicable | Subject: Subject 01
Received: 2022/05/1 | Accepted: 2022/09/20 | Published: 2022/11/19