The effect of salicylic acid foliar application on the expression pattern of genes encoding antioxidant enzymes of spring bread wheat (Triticum aestivum L.) under water deficit conditions

khalili, marouf; ,; ,

Volume 18, Issue 2 (10-2023) MGj 2023, 18(2): 211-224 | Back to browse issues page

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khalili M. The effect of salicylic acid foliar application on the expression pattern of genes encoding antioxidant enzymes of spring bread wheat (Triticum aestivum L.) under water deficit conditions. , MGj. 2023; 18 (2) : 9
URL: http://mg.genetics.ir/article-1-1808-en.html

The effect of salicylic acid foliar application on the expression pattern of genes encoding antioxidant enzymes of spring bread wheat (Triticum aestivum L.) under water deficit conditions

Marouf Khalili ^*

Associate Professor, Department of Biotechnology and Plant Breeding, Payame Noor University, Tehran, Iran

Abstract: (1744 Views)

To investigate the expression pattern of genes encoding antioxidant enzymes under water deficit stress in spring bread wheat, a factorial experiment was conducted in a randomized complete block design in 2020. In this study, agronomic traits, enzyme-related traits, antioxidant activity as well as the amount of gene expression affected by irrigation and salicylic acid were measured. In this study, there was a significant difference between irrigation levels and salicylic acid in terms of the effect on all traits. There was a significant difference between interaction treatments in terms of the effect on all traits except spike length and 1000-kernel weight. Mean comparison of interaction treatments showed that foliar spraying of 1 mM salicylic acid under 100% plant water requirement treatment had the highest plant height (64.76 cm), number of tillers per plant (3.71), number of seeds per spike (21.2) Dry matter yield (15.14 g / plant) grain yield (10.68 g / plant), chlorophyll an (8.66 mg / g fresh weight), chlorophyll b (2.66 mg / g fresh weight), Carotenoids (4.13 mg / g wet weight). While the highest content of leaf proline (0.53 mg / g fresh weight), leaf phenol (Galic acid / 82 DW9 / 82), catalase activity (97.09 mMolH2O2 / min mg protein), ascorbate peroxidase (mMolH₂O₂) / min mg protein 03/120), superoxide dismutase (mMolH2O2 / min mg protein 89/330) was allocated to foliar application of 1 mg salicylic acid under 50% plant water requirement. In this study, water deficit stress and salicylic acid foliar application had a synergistic effect on increasing the activity of antioxidant enzymes. The highest expression of superoxide dismutase, polyphenol oxidase, and catalase genes was observed by foliar application of 1 and 2 mM salicylic acid, and the highest expression of ascorbate peroxidase gene was observed by 1 mM foliar application of salicylic acid under 50% plant water requirement treatment. Application of salicylic acid under irrigation 50 and 75% of plant water requirement increased the expression of antioxidant genes in comparison with salicylic acid control treatment in each plant water require treatments and under 100% plant water requirement.

Article number: 9

Keywords: Superoxide dismutase, Grain yield, Chlorophyll, water deficit

Full-Text [PDF 853 kb] (637 Downloads)

Type of Study: Applicable | Subject: Subject 01
Received: 2023/02/5 | Accepted: 2023/06/8 | Published: 2023/09/24