Volume 19, Issue 3 (12-2024)
MGj 2024, 19(3): 147-158 |
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Valizadeh M, Heidari Japelaghi R, Haddad R, Dorani-Uliaie E, Jalali-Javaran M. Molecular Farming: Factors Affecting in Increasing the Expression Level of Recombinant Protein in Plants. MGj 2024; 19 (3) : 1
URL: http://mg.genetics.ir/article-1-1822-en.html
URL: http://mg.genetics.ir/article-1-1822-en.html
Mostafa Valizadeh * 
, Reza Heidari Japelaghi , Raheem Haddad , Ebrahim Dorani-Uliaie , Mokhtar Jalali-Javaran
, Reza Heidari Japelaghi , Raheem Haddad , Ebrahim Dorani-Uliaie , Mokhtar Jalali-Javaran
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Abstract: (249 Views)
Currently, commercial systems for recombinant protein production rely on bacteria, yeasts, insects, and mammalian cell cultures. While each of these systems offers distinct advantages, their widespread application is often constrained by scalability challenges, high costs, and safety concerns. In recent years, plants have emerged as promising bioreactors for recombinant protein production, driven by their low production costs, enhanced product safety, and ease of scalability.
Plant-based systems have successfully produced a variety of recombinant products, including vaccine antigens, pharmaceutical proteins, industrial enzymes, nutritional supplements, and biopolymers. Notably, several plant-derived recombinant proteins have advanced to late-stage clinical trials, and some have already been commercialized. However, the effective use of plants as bioreactors hinges on achieving high and stable protein accumulation levels throughout the plant's life cycle and across subsequent generations. To address these challenges, various strategies have been developed to enhance protein expression in transgenic plants. These include tissue-specific transcription enhancement, increased transcript stability, tissue-specific protein targeting, translation optimization, and improved protein stability and subcellular accumulation. This review discusses the factors influencing recombinant protein expression in plant-based systems, highlighting recent advancements and strategies for improving yield and stability to realize the full potential of plants as scalable and cost-effective bioreactors.
Plant-based systems have successfully produced a variety of recombinant products, including vaccine antigens, pharmaceutical proteins, industrial enzymes, nutritional supplements, and biopolymers. Notably, several plant-derived recombinant proteins have advanced to late-stage clinical trials, and some have already been commercialized. However, the effective use of plants as bioreactors hinges on achieving high and stable protein accumulation levels throughout the plant's life cycle and across subsequent generations. To address these challenges, various strategies have been developed to enhance protein expression in transgenic plants. These include tissue-specific transcription enhancement, increased transcript stability, tissue-specific protein targeting, translation optimization, and improved protein stability and subcellular accumulation. This review discusses the factors influencing recombinant protein expression in plant-based systems, highlighting recent advancements and strategies for improving yield and stability to realize the full potential of plants as scalable and cost-effective bioreactors.
Article number: 1
Type of Study: review |
Subject:
Subject 01
Received: 2023/05/25 | Accepted: 2024/12/14 | Published: 2025/01/14
Received: 2023/05/25 | Accepted: 2024/12/14 | Published: 2025/01/14
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