A comprehensive analysis of the Snakin/GASA gene family: insights into their origin, expansion, diversity and expression

Panji, Anahita; Ismaili, Ahmad; Sohrabi, Seyyed Mohsen

Volume 19, Issue 2 (10-2024) MGj 2024, 19(2): 75-89 | Back to browse issues page

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Panji A, Ismaili A, Sohrabi S M. A comprehensive analysis of the Snakin/GASA gene family: insights into their origin, expansion, diversity and expression. MGj 2024; 19 (2) : 1
URL: http://mg.genetics.ir/article-1-1837-en.html

A comprehensive analysis of the Snakin/GASA gene family: insights into their origin, expansion, diversity and expression

Anahita Panji

, Ahmad Ismaili ^*

, Seyyed Mohsen Sohrabi

Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract: (319 Views)

The Snakin/GASA family consists of small, cysteine-rich peptides that are widely distributed across plant genomes, playing crucial roles in plant growth, development, and responses to biotic and abiotic stresses. In this study, the presence of the GASA gene family across various organisms was examined, and structural and expression variations of these genes were compared in different plant species. Additionally, post-translational modifications and the antimicrobial properties of GASA proteins were predicted to provide further insight into their regulatory mechanisms. Key characteristics observed for all GASA genes included the presence of the GASA domain, signal peptides, extracellular accumulation, six disulfide bridges, post-translational phosphorylation, and antimicrobial activity. Differential expression patterns were noted in various tissues (logFPKM = 0-2.5), with induction under biotic, abiotic, and hormonal stresses (logFold Change ranging from -10 to +5, particularly under drought and heat conditions). Furthermore, key cis-regulatory elements were identified in the promoter regions of these genes, highlighting the diverse roles and functions of the Snakin/GASA gene family. Physicochemical analysis revealed that most of the studied GASA proteins were unstable and hydrophobic. A strong interaction was also predicted between the GASA gene family and transcription factors from the Dof, ERF, and MYB families in both Arabidopsis and rice. Overall, these findings provide valuable insights into the functional diversity of GASA genes and offer a foundation for future research.

Article number: 1

Keywords: Biotic and abiotic stresses, Genomic Analysis, Monocot and Dicot plants, Snakin/GASA gene family, Tissue-specific expression

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Type of Study: Applicable | Subject: Subject 01
Received: 2023/09/3 | Accepted: 2024/08/24 | Published: 2024/11/16