Volume 15, Issue 4 (1-2021)
MGj 2021, 15(4): 317-326 |
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Shirzad H, Ahmadi J, Jafaraghaei M, Sorkhi B. Genetic variation of high molecular weight glutenin subunits (HMWGS) in Aegilops triuncialis L. accessions collected from northern regions of Iran. MGj 2021; 15 (4) :317-326
URL: http://mg.genetics.ir/article-1-104-en.html
URL: http://mg.genetics.ir/article-1-104-en.html
Department of Plant Genetic Engineering, College of Agriculture, Zanjan University, Zanjan, I.R.Iran
Abstract: (1498 Views)
Genetic diversity is the basis of plant breeding that allows selection and improvement of plants with desirable traits and characteristics. Aegilops spp. is one of the wild relatives of bread wheat and has a large distribution in the Middle East and West Asia, that Iran covering a large part of this region. In this study, 75 accessions of Aegilops triuncialis species, stored in the National Genetic Bank of Iran, were evaluated for the diversity of high molecular weight glutenin subunits (HMW-GS) using SDS-PAGE method. Generally 10 protein bands in 20 different patterns were observed among the samples. The highest band frequency was for bands h and i with frequency of 0.93 and the least frequency was related to band k with frequency of 0.026 that was observed only in Qazvin and Golestan provinces. The highest corrected genetic variation (uh) was observed in Markazi province (0.4) and the least corrected genetic variation was for Kurdistan province (0.13). The genetic distance matrix of the provinces based on Nei index showed that the highest distance was between Golestan and North Khorasan provinces with West Azarbaijan province, and the lowest genetic distances observed between Khorasan Razavi and Semnan provinces as well as North Khorasan and Mazandaran provinces. Cluster analysis using UPGMA method divides the studied provinces into three groups. There was a clear logical relationship between genetic diversity and geographical variation.
Type of Study: Applicable |
Subject:
Subject 01
Received: 2019/10/7 | Accepted: 2021/01/5 | Published: 2021/02/2
Received: 2019/10/7 | Accepted: 2021/01/5 | Published: 2021/02/2
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