Volume 15, Issue 2 (10-2020)                   MGj 2020, 15(2): 171-181 | Back to browse issues page

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shahraki H, mahdi nezhad N, fakheri B, haddadi F. Separation and identification of FEH1 gene in thorny artichoke plant (Cynara cardunculus) and its relative expression under the influence of abiotic stresses. MGj. 2020; 15 (2) :171-181
URL: http://mg.genetics.ir/article-1-1643-en.html
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Abstract:   (689 Views)
Cynara cardunculus L is a diploid herbaceous plant and perennial. Artichoke sugar is a fructan that has the least effect on blood sugar and is very useful for diabetics in this regard. The aim of this study was to isolate, identify, and evaluate the relative expression of the fructan 1-exhohydrolase gene (FEH1) gene in the artichoke plant. In order to identify and evaluate the molecular evolution of FEH1 gene, DNA was extracted from leaf samples of artichoke and after amplification was sequenced by PCR technique. Also in order to investigate the effects of salinity, silver nanoparticles of green synthesis and salicylic acid on the change of relative expression of FEH1 gene, an experiment was performed as a factorial in a completely randomized design in a greenhouse. The relative expression pattern of this gene was measured by Real Time PCR. The results of analysis and analysis of nucleotide sequences of the FEH1 gene showed that the highest frequency belonged to Guanin (31.56) and the lowest frequency belonged to Tymien base(12.4). The results of the Neutrality test also showed a directional choice on this gene during evolution. A phylogenetic study of the FEH-1 gene sequence and its comparison with other similar sequences in different plants proved that the similarity between the studied sequences was high(more 90%) and the plants were slightly different from each other in terms of genetic distance. The results of comparing the mean interactions of salicylic acid and salinity concentrations showed that the application of 12  ds m-1of salinity alone significantly increased the expression of FEH1 gene at the probability level of one percent. The use of salicylic acid in combination with salinity moderated the effects of salinity and also reduced FEH1 gene expression. Also, the results of comparing the average interaction of salicylic acid and nano silver concentrations showed that in the treatment of 200 and 400 mM salicylic acid with 40 mM nano silver at the probability level 1% expression of FEH1 gene in artichoke plant increased significantly. Clearly, it was found that both in terms of expression and expression intensity of FEH1 gene, there is a high potential to focus on increasing soluble sugars and ultimately increase plant tolerance to salinity stress in artichoke .
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Type of Study: Applicable | Subject: Subject 01
Received: 2020/05/15 | Accepted: 2020/10/18 | Published: 2020/11/1

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