Volume 18, Issue 2 (10-2023)
MGj 2023, 18(2): 199-210 |
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Kiani Deh Kian M, Taheri H, Sadr A S. Bioinformatics evaluation of the gene expression profile of Arabidopsis seedlings in order to identify heat stress responsive hob genes. MGj 2023; 18 (2) : 8
URL: http://mg.genetics.ir/article-1-1803-en.html
URL: http://mg.genetics.ir/article-1-1803-en.html
Agricultural Sciences and Natural Resources University of Khuzestan
Abstract: (397 Views)
High-temperature stress is an environmental challenge limiting plant productivity and growth. Plants have evolved various mechanisms to respond to heat stress. In this study, to explore the molecular aspects and functional analysis of heat tolerance, the microarray data of the expression profile of heat stress in Arabidopsis seedlings were retrieved from the gene expression omnibus database (GEO) database with accession number GSE103398. After quality control and data normalization, the Limma package was used to assess the differentially expressed genes (DEGs). The 7780 heat responsive DEGs were identified with the criteria of p-value <0.01 and |log2Fold change| > 2 which were further analyzed by bioinformatics tools for gene ontology (GO) classification, KEGG pathways analysis, protein-protein interaction networks and potential hob genes. For the biological process (BP), the most enriched GO terms in heat stress responsive DEGs were related to the regulation of transcription, oxidation-reduction process and response to abscisic acid. Based on the KEGG enrichment metabolic pathway, plant hormone signal transduction, biosynthesis of secondary metabolites and glycerophospholipid metabolism pathways were found to be majorly affected under heat stress. Among the identified candidate–hub genes with high connectivity in modules, Auxin Response Factor 5/MONOPTROS (ARF5/MP), TIR1 (transport inhibitor response1) as auxin receptor, Arabidopsis Histidine Kinase 4 (AHK4)/ WOL(Wooden Leg) as cytokinin receptor, SDP1-LIKE (Sugar –dependent1 –like) involved in TAG degradation, 2-lysophosphatidic acid transferase 2/4 (LPAT2/4) involved in TAG biosynthesis and pyrroline-5-carboxylate synthetase1/2 (P5CS1/2) involved in proline biosynthesis were introduced as genes playing critical roles in responding to heat stress. Function analysis of the hob genes disclosed that the acclimatization responses to heat stress were mainly associated with control of root and shoot apical meristem activity, hypocotyl elongation, vascular tissues development, heat shock protein activation, changes in the membrane lipid composition and cellular redox homeostasis. Therefore, breeding strategies must be developed to enhance heat tolerance by manipulating the components involved in these pathways.
Article number: 8
Type of Study: Applicable |
Subject:
Subject 01
Received: 2023/01/19 | Accepted: 2023/06/25 | Published: 2023/09/24
Received: 2023/01/19 | Accepted: 2023/06/25 | Published: 2023/09/24
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