Volume 20, Issue 1 (10-2025)
MGj 2025, 20(1): 47-57 |
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Sedaghat F, Habibollahi H, Safari Motlagh M R. Investigating the antimicrobial effect of Ginseng and Artichoke plant extracts and changes in sea enterotoxin gene expression in Staphylococcus aureus. MGj 2025; 20 (1) : 5
URL: http://mg.genetics.ir/article-1-1816-en.html
URL: http://mg.genetics.ir/article-1-1816-en.html
Department of Plant Protection, Rasht Branch, Islamic Azad University. Rasht, Iran.
Abstract: (171 Views)
Increasing resistance of Staphylococcus aureus to antibiotics has prompted the search for alternative herbal medicines. Both artichoke and ginseng contain bioactive phenolic compounds with antibacterial properties. This research studied two strains of S. aureus: a methicillin-resistant strain (MRSA) and a ciprofloxacin-sensitive strain with multiple antibiotic resistance (standard strain ATCC25923 and a pathogenic strain from patient skin), both carrying the enterotoxin sea gene. Antimicrobial activity of the extracts was assessed using disk diffusion, followed by determination of the minimum inhibitory concentration (MIC). SubMIC concentrations (2500 µg/ml) were used to evaluate the impact of the extracts on sea gene expression. RNA extraction, cDNA synthesis, and Real-Time PCR were conducted for analysis. The results showed that both extracts inhibited S. aureus growth, with the combined extracts producing larger non-growth zones (20 mm for the pathogen and 19 mm for the standard strain). The MIC for the extracts and their combination was 5000 µg/ml. Treatment with ginseng extract reduced sea gene expression in the pathogen strain to 8% of control, while artichoke extract reduced it to 60%. Combined treatment lowered expression to 3%. In the standard strain, ginseng decreased gene expression to 0.03%, and artichoke completely inhibited it. The synergistic effects of ginseng and artichoke led to a significant reduction (p < 0.05) in sea gene expression, suggesting these extracts could effectively reduce pathogenicity and symptoms associated with S. aureus by suppressing enterotoxin A gene expression.
Article number: 5
Type of Study: Applicable |
Subject:
Subject 03
Received: 2023/04/21 | Accepted: 2025/07/21 | Published: 2025/10/7
Received: 2023/04/21 | Accepted: 2025/07/21 | Published: 2025/10/7
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