Volume 13, Issue 2 (7-2018)                   MGj 2018, 13(2): 301-306 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Saaed Mocheshi K, Izadi Darbandi A, Saaed Mocheshi N. Introduction of an applied device for DNA purification from Agarose gel: purification and cloning of HMGR gene from American ginseng plant. MGj 2018; 13 (2) :301-306
URL: http://mg.genetics.ir/article-1-66-en.html
Abstract:   (1415 Views)
In order to gene cloning, at first desired gene should separate from other contaminants (proteins, primer-dimer bands unskilled, etc.). Various physical and chemical methods were used for purification of target gene from other contaminates; Here we have applied a new physical methods for purification of gen from agarose gel under an electric field containing TAE.1X fluid. We isolated PqHMGR form Panax quinquefoliusby using specific primers based on cDNA sequences. Ligation of isolated gene tovector and its cloning was done through E.coli (DH5α). Finally, PqHMGR gene was purified by the foresaid device and after cloning in desired vector, extracted plasmid were sequenced for confirmation of gene purification and cloning. Our resultd showed accuracy of this device in gene purification.
Full-Text [PDF 378 kb]   (1062 Downloads)    
Type of Study: Research | Subject: Subject 01
Received: 2016/10/30 | Accepted: 2018/10/7 | Published: 2019/10/1

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | فصلنامه علمی ژنتیک نوین

Designed & Developed by : Yektaweb