Volume 13, Issue 2 (7-2018)                   MGj 2018, 13(2): 301-306 | Back to browse issues page

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Saaed Mocheshi K, Izadi Darbandi A, Saaed Mocheshi N. Introduction of an applied device for DNA purification from Agarose gel: purification and cloning of HMGR gene from American ginseng plant. MGj. 2018; 13 (2) :301-306
URL: http://mg.genetics.ir/article-1-66-en.html
Abstract:   (694 Views)
In order to gene cloning, at first desired gene should separate from other contaminants (proteins, primer-dimer bands unskilled, etc.). Various physical and chemical methods were used for purification of target gene from other contaminates; Here we have applied a new physical methods for purification of gen from agarose gel under an electric field containing TAE.1X fluid. We isolated PqHMGR form Panax quinquefoliusby using specific primers based on cDNA sequences. Ligation of isolated gene tovector and its cloning was done through E.coli (DH5α). Finally, PqHMGR gene was purified by the foresaid device and after cloning in desired vector, extracted plasmid were sequenced for confirmation of gene purification and cloning. Our resultd showed accuracy of this device in gene purification.
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Type of Study: Research | Subject: Subject 01
Received: 2016/10/30 | Accepted: 2018/10/7 | Published: 2019/10/1

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